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We describe a simple and reliable method for differential immunolabeling of pre- and post-synaptic signal peptides at the ultrastructural level. Hypothalamic tissues of rats, including the suprachiasmatic nucleus, were cut on a Vibratome. Visualization of the immunolabeling of somatostatin (SRIH) and vasoactive intestinal polypeptide (VIP) was performed with avidin-biotin-peroxidase-diaminobenzidine (DAB). The end product of the DAB to VIP was further silver-intensified in a physical processing using silver nitrate, and the silver grains were finally substituted for gold. DAB-labeled SRIH fibers synapse on gold-labeled VIP perikarya and dendrites in the suprachiasmatic nucleus.
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
January 2000,
Methods in molecular medicine,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
November 2005,
Chromosoma,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
January 2023,
Methods in molecular biology (Clifton, N.J.),
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
April 2008,
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
January 2008,
Methods in cell biology,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
July 2012,
Cold Spring Harbor protocols,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
July 2012,
Cold Spring Harbor protocols,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
January 2013,
Methods in molecular biology (Clifton, N.J.),
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
October 2014,
Histochemistry and cell biology,
M Maegawa, and
S Hisano, and
Y Tsuruo, and
S Katoh, and
J Nakanishi, and
M Chikamori-Aoyama, and
S Daikoku
July 1998,
Microscopy research and technique,
