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A simple and specific assay to measure the activity of two coenzyme A derivative-processing enzymes, i.e., phosphotransacetylase (EC 2.3.1.8) and acetyl-coenzyme A carboxylase (EC 6.4.1.2), is described. The assay is based on the HPLC analysis of the short-chain coenzyme A derivatives formed by the enzymatic reaction, viz., acetyl-CoA and malonyl-CoA. For this purpose, ion-pair reversed-phase HPLC conditions are optimized. Furthermore, the influence of several variables on the enzyme reaction is studied in order to get maximum activity. Due to its short analysis time, good selectivity, and chromatogram information, HPLC proves to be an excellent method for the assay of these enzymes.
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
January 1988,
Gene analysis techniques,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
April 2011,
Analytical biochemistry,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
June 2013,
Analytical biochemistry,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
November 1980,
Journal of chromatography,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
January 2013,
Se pu = Chinese journal of chromatography,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
January 1974,
Current topics in cellular regulation,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
February 2006,
Analytical biochemistry,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
July 1951,
The Journal of biological chemistry,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
June 2019,
Electrophoresis,
B De Spiegeleer, and
G Mannens, and
G Slegers, and
W Van den Bossche, and
A Claeys
November 2012,
Analytical biochemistry,
