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The light subunit of mushroom, Agaricus bisporus, tyrosinase (LSMT), has been identified as an extrinsic component of the enzyme. Its function is unknown, but it can cross an epithelial cell layer, which suggests that it can be absorbed by the intestine. A similar capability has been demonstrated for the HA-33 component of the progenitor toxin from Clostridium botulinum, which is the closest structural homolog of LSMT. Unlike HA-33, LSMT appears to be non-immunogenic as shown by preliminary tests in Swiss Webster mice. We investigated the immunogenicity and histopathology of LSMT in mice to determine its safety in vivo. LSMT did not evoke generation of antibodies after prolonged periods of intraperitoneal administration. Histopathological observations confirmed the absence of responses in organs after twelve weekly administrations of LSMT. We found that LSMT is not toxic and is less immunogenic than the C. botulinum HA-33 protein, which supports further research and development for pharmaceutical application.
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
September 2017,
International journal of biological macromolecules,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
April 1976,
Research communications in chemical pathology and pharmacology,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
February 1977,
Pharmacological research communications,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
March 1964,
Nature,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
January 1988,
Experimental gerontology,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
January 1986,
Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
December 1988,
The Indian journal of medical research,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
January 1985,
Journal of environmental pathology, toxicology and oncology : official organ of the International Society for Environmental Toxicology and Cancer,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
February 1979,
Biochemical Society transactions,
W T Ismaya, and
A Efthyani, and
D S Retnoningrum, and
X Lai, and
B W Dijkstra, and
R R Tjandrawinata, and
H Rachmawati
April 1998,
The Biochemical journal,
