BIOMAT Database Logo BIOMAT Database Logo

Mapping of DNA markers close to the fragile site on the human X chromosome at Xq27.3. 1987

M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies

We report the identification of a new RFLP detected by the DNA probe MN12, which is linked to both the fragile site on the X chromosome at Xq27.3 and the highly polymorphic locus detected by St14 (DXS52). In situ mapping confirms the localisation of MN12 distal to the fragile site. A detailed physical analysis of this region of the X chromosome using pulsed-field gel electrophoresis has shown that MN12, St14 and DX13 (DXS15) are physically linked within a region of 470kb. A long range restriction map around the MN12 locus reveals at least two candidate HTF islands, suggesting the existence of expressed sequences in this region.

UI MeSH Term Description Entries
D008297 Male Males
D009693 Nucleic Acid Hybridization Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503) Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D009710 Nucleotide Mapping Two-dimensional separation and analysis of nucleotides. Fingerprints, Nucleotide,Fingerprint, Nucleotide,Mapping, Nucleotide,Mappings, Nucleotide,Nucleotide Fingerprint,Nucleotide Fingerprints,Nucleotide Mappings
D010375 Pedigree The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition. Family Tree,Genealogical Tree,Genealogic Tree,Genetic Identity,Identity, Genetic,Family Trees,Genealogic Trees,Genealogical Trees,Genetic Identities,Identities, Genetic,Tree, Family,Tree, Genealogic,Tree, Genealogical,Trees, Family,Trees, Genealogic,Trees, Genealogical
D010957 Plasmids Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS. Episomes,Episome,Plasmid
D011110 Polymorphism, Genetic The regular and simultaneous occurrence in a single interbreeding population of two or more discontinuous genotypes. The concept includes differences in genotypes ranging in size from a single nucleotide site (POLYMORPHISM, SINGLE NUCLEOTIDE) to large nucleotide sequences visible at a chromosomal level. Gene Polymorphism,Genetic Polymorphism,Polymorphism (Genetics),Genetic Polymorphisms,Gene Polymorphisms,Polymorphism, Gene,Polymorphisms (Genetics),Polymorphisms, Gene,Polymorphisms, Genetic
D012150 Polymorphism, Restriction Fragment Length Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment. RFLP,Restriction Fragment Length Polymorphism,RFLPs,Restriction Fragment Length Polymorphisms
D002873 Chromosome Fragility Susceptibility of chromosomes to breakage leading to translocation; CHROMOSOME INVERSION; SEQUENCE DELETION; or other CHROMOSOME BREAKAGE related aberrations. Chromosomal Fragility,Fragility, Chromosomal,Fragility, Chromosome
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man

Related Publications

M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Genetic and physical mapping of a novel region close to the fragile X site on the human X chromosome.
May 1989, Genomics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
The human genes for hemophilia A and hemophilia B flank the X chromosome fragile site at Xq27.3.
March 1985, The EMBO journal,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Linear order of new and established DNA markers around the fragile site at Xq27.3.
May 1991, Genomics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Genetic mapping of DNA segments relative to the locus for the fragile-X syndrome at Xq27.3.
May 1985, American journal of human genetics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
DNA studies of X-linked mental retardation associated with a fragile site at Xq27.3.
January 1987, Upsala journal of medical sciences. Supplement,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Pulsed-field gel mapping studies in the vicinity of the fragile site at Xq27.3.
January 1988, American journal of medical genetics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Fragile X expression and X inactivation. II. The fragile site at Xq27.3 has a basic function in the pathogenesis of fragile X-linked mental retardation.
August 1991, Human genetics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Effects of hyperoxia and caffeine on the expression of fragile site at Xq27.3.
February 1996, American journal of medical genetics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Concurrence of the triple-X syndrome and expression of the fragile site Xq27.3.
March 1988, Human genetics,
M Patterson, and S Kenwrick, and S Thibodeau, and K Faulk, and M G Mattei, and J F Mattei, and K E Davies
Fragile X expression and X inactivation. I. The expression of the fragile site at Xq27.3 is not suppressed on inactive X chromosomes separated from the active homologue.
October 1990, Human genetics,
Copied contents to your clipboard!