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In glucocorticosteroid-treated diabetic rats, glutamine synthetase enzyme levels in the liver are decreased 3-fold, whereas carbamoylphosphate synthetase enzyme levels are increased 2.3-fold. In addition, immunohistochemistry shows that under these conditions the distribution of carbamoylphosphate synthetase is expanded over the entire liver acinus, whereas that of glutamine synthetase is reduced to very few cells bordering the central (terminal hepatic) veins. Using a newly isolated cDNA complementary to rat liver glutamine synthetase mRNA, we show that this regulation is primarily effected at a pretranslational level. (For data on carbamoylphosphate synthetase mRNA levels, see De Groot et al. (1986) Biochim. Biophys. Acta 866, 61-67). Furthermore, hybridization studies show stimulatory effects of both glucocorticosteroids and thyroid hormone on the glutamine synthetase mRNA level. Attempts to localize glutamine synthetase mRNA within the liver acinus by selective destruction of the pericentral zone failed because of generally low levels of liver mRNAs after CCl4 poisoning. In contrast to the situation after birth, significantly higher glutamine synthetase mRNA/enzyme activity ratios in fetal rat liver point to the presence of additional post-transcriptional control mechanisms before birth. These findings complement similar observations on carbamoylphosphate synthetase gene expression (De Groot et al. (1986) Biochim. Biophys. Acta 866, 61-67).
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
July 1988,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
February 1990,
Proceedings of the National Academy of Sciences of the United States of America,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
November 1984,
Biochemical and biophysical research communications,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
December 1997,
Histochemistry and cell biology,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
December 1977,
Biochemical and biophysical research communications,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
January 1987,
Pediatric pathology,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
January 2008,
Cells, tissues, organs,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
October 1990,
Hepatology (Baltimore, Md.),
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
December 1991,
European journal of cell biology,
C J de Groot, and
G H ten Voorde, and
R E van Andel, and
A te Kortschot, and
J W Gaasbeek Janzen, and
R H Wilson, and
A F Moorman, and
R Charles, and
W H Lamers
December 2001,
Cell and tissue research,
