OBJECTIVE To describe the clinical application of a vascularized preputial free flap in the reconstruction of a large facial-labial defect. METHODS Case report. METHODS An adult neutered male mongrel dog with a large left facial-labial defect. METHODS A preputial free flap was created by elevating the cranial part of the prepuce, using the ipsilateral caudal superficial epigastric vessels as a vascular pedicle. The flap was transferred to the facial-labial defect and the donor vessels anastomosed to the sublingual artery and vein, using standard microvascular technique. RESULTS The free flap survived, leading to good cosmetic and functional outcomes. No major complications were encountered at either the donor or recipient sites after 2 years of follow-up. CONCLUSIONS Vascularized preputial free flaps can be used to manage composite facial and oral cavity defects involving large areas of skin and mucosa.