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Thy-1 is a cell surface differentiation marker which shows distinct patterns of tissue-specific expression in different species. In man, the Thy-1 antigen is encoded by chromosome 11. We have examined the regulatory signals determining human Thy-1 expression through serologic analysis of rodent-human somatic cell hybrids retaining human chromosome 11 in which the fusion partners belong to distinct differentiation lineages. Cell surface expression of human Thy-1 was determined by mixed hemadsorption assays with two monoclonal antibodies (mAb), K117 and L127, shown to detect authentic human Thy-1 through analysis of COS-7 monkey kidney cells transfected with a cloned human Thy-1 gene. Three different patterns of human Thy-1 expression were observed when hybrid cells, constructed with different human and rodent cell types, were tested with mAb K117 and L127. Hybrids formed between Thy-1+ human neuroblastoma cells and Thy-1- mouse neuroblastoma cells, or hybrids between Thy-1+ human fibroblasts and the Thy-1- mouse kidney carcinoma, RAG, retain human Thy-1 expression. In contrast, hybrids formed between either Thy-1+ human neuroblastoma cells or Thy-1+ human fibroblasts and Thy-1- mouse L cells lose expression of human Thy-1 even though chromosome 11 is retained. Finally, hybrids formed between Thy-1- human peripheral lymphocytes or a Thy-1- lymphoblastoid B cell line and Thy-1- Chinese hamster fibroblasts begin to express human Thy-1. These studies suggest that both positive and negative trans-acting signals may play a role in the tissue-specific regulation of the human Thy-1 gene.
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
July 1984,
Somatic cell and molecular genetics,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1981,
Immunogenetics,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
June 1985,
Biochemical genetics,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1974,
Annual review of genetics,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1989,
Immunology series,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
November 1979,
The Journal of general virology,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1983,
Progress in clinical and biological research,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1980,
Cytogenetics and cell genetics,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1978,
Cytogenetics and cell genetics,
W J Rettig, and
H Nishimura, and
A K Yenamandra, and
T Seki, and
F Obata, and
H R Beresford, and
L J Old, and
J Silver
January 1976,
Cytogenetics and cell genetics,
