The effects of trypanocidal drugs on Trypanosoma b. brucei infections in Glossina m. centralis have been investigated. Pentamidine and suramin exhibited no significant effects but both berenil and samorin reduced the number of salivary gland infections in comparison with controls. Berenil at concentrations of 10, 1.0 and 0.1 microgram/ml significantly reduced the number of mature infections when fed to flies throughout the whole period of trypanosome development. A similar result was obtained with samorin at 0.1 microgram/ml. Subsequent experiments showed that administration of both drugs at an early stage was more effective in preventing the maturation of infection than a later but more prolonged administration. Reported drug levels in the blood of different experimental host animals are of the same magnitude as those used here. It is suggested that repeated feeding of T. b. brucei infected Glossina on drug-treated hosts may reduce transmission, although alternative bloodmeal sources would reduce this effect. These influences are worthy of investigation in the field.