BIOMAT Database Logo BIOMAT Database Logo

Proteomic Analysis of the Secretome of Porcine Alveolar Macrophages Infected with Porcine Reproductive and Respiratory Syndrome Virus. 2017

Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, Shanghai, PR China.

Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome (PRRS), which is characterized by reproductive failure and respiratory disorders. The secretome of PRRSV-infected porcine alveolar macrophages (PAMs), which are the primary target cells of PRRSV, was analyzed by label-free quantitative proteomics to gain a profile of proteins secreted during PRRSV infection. A total of 95 secreted proteins with differentially expressed levels between PRRSV- and mock-infected PAMs was screened. Among these, the expression levels of 49 and 46 proteins were up-regulated and down-regulated, respectively, in PRRSV-infected cell supernatants, as compared with mock-infected cell supernatants. Bioinformatic analysis revealed that the differentially expressed proteins were enriched in several signaling pathways related to the immune and inflammatory responses, such as the Toll-like receptor signaling pathway and NF-kappa B signaling pathway, and involved in a great diversity of biological processes, such as protein binding and localization, as well as immune effector processes. In addition, PRRSV-infected cell supernatants induced significant expression of inflammatory cytokines in vascular endothelial cells. These findings suggest that the secreted proteins play potential roles in the host immune and inflammatory responses as well as PRRSV replication, thereby providing new insights into cell-to-cell communication during PRRSV infection.

UI MeSH Term Description Entries
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D015398 Signal Transduction The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway. Cell Signaling,Receptor-Mediated Signal Transduction,Signal Pathways,Receptor Mediated Signal Transduction,Signal Transduction Pathways,Signal Transduction Systems,Pathway, Signal,Pathway, Signal Transduction,Pathways, Signal,Pathways, Signal Transduction,Receptor-Mediated Signal Transductions,Signal Pathway,Signal Transduction Pathway,Signal Transduction System,Signal Transduction, Receptor-Mediated,Signal Transductions,Signal Transductions, Receptor-Mediated,System, Signal Transduction,Systems, Signal Transduction,Transduction, Signal,Transductions, Signal
D016207 Cytokines Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner. Cytokine
D016676 Macrophages, Alveolar Round, granular, mononuclear phagocytes found in the alveoli of the lungs. They ingest small inhaled particles resulting in degradation and presentation of the antigen to immunocompetent cells. Alveolar Macrophages,Macrophages, Pulmonary,Pulmonary Macrophages,Macrophage, Pulmonary,Pulmonary Macrophage,Alveolar Macrophage,Macrophage, Alveolar
D054884 Host-Pathogen Interactions The interactions between a host and a pathogen, usually resulting in disease. Host Pathogen Interaction,Host-Pathogen Relations,Pathogen-Host Interaction,Pathogen-Host Interactions,Host Pathogen Interactions,Host Pathogen Relations,Host-Pathogen Interaction,Host-Pathogen Relation,Interaction, Host Pathogen,Interaction, Host-Pathogen,Interaction, Pathogen-Host,Interactions, Host Pathogen,Interactions, Host-Pathogen,Interactions, Pathogen-Host,Pathogen Host Interaction,Pathogen Host Interactions,Pathogen Interaction, Host,Pathogen Interactions, Host,Relation, Host-Pathogen,Relations, Host-Pathogen
D019316 Porcine respiratory and reproductive syndrome virus A species of ARTERIVIRUS causing reproductive and respiratory disease in pigs. The European strain is called Lelystad virus. Airborne transmission is common. Lelystad Virus,PRRSV,Swine Infertility and Respiratory Syndrome Virus,Porcine Reproductive and Respiratory Syndrome Virus,Swine Infertility Respiratory Syndrome Virus
D019318 Porcine Reproductive and Respiratory Syndrome A syndrome characterized by outbreaks of late term abortions, high numbers of stillbirths and mummified or weak newborn piglets, and respiratory disease in young unweaned and weaned pigs. It is caused by PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS. (Radostits et al., Veterinary Medicine, 8th ed, p1048) Blue-Eared Pig Disease,Mystery Swine Disease,PRRS,Porcine Epidemic Abortion and Respiratory Syndrome,Swine Infertility and Respiratory Syndrome,Blue Eared Pig Disease,Pig Disease, Blue-Eared,Swine Disease, Mystery
D020543 Proteome The protein complement of an organism coded for by its genome. Proteomes

Related Publications

Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Reactomes of porcine alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.
January 2013, PloS one,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Cytokine production in immortalized porcine alveolar macrophages infected with porcine reproductive and respiratory syndrome virus.
December 2012, Veterinary immunology and immunopathology,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
SILAC-based quantitative proteomic analysis of secretome of Marc-145 cells infected with porcine reproductive and respiratory syndrome virus.
October 2016, Proteomics,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Unveiling the long non-coding RNA profile of porcine reproductive and respiratory syndrome virus-infected porcine alveolar macrophages.
March 2021, BMC genomics,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Cryptotanshinone protects porcine alveolar macrophages from infection with porcine reproductive and respiratory syndrome virus.
November 2020, Antiviral research,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Ivermectin inhibits porcine reproductive and respiratory syndrome virus in cultured porcine alveolar macrophages.
February 2016, Archives of virology,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Factors affecting the permissiveness of porcine alveolar macrophages for porcine reproductive and respiratory syndrome virus.
January 2009, Archives of virology,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Proteomic analysis of porcine alveolar macrophages infected with porcine circovirus type 2.
June 2012, Journal of proteomics,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
Immune Molecules' mRNA Expression in Porcine Alveolar Macrophages Co-Infected with Porcine Reproductive and Respiratory Syndrome Virus and Porcine Circovirus Type 2.
March 2023, Viruses,
Yuming Li, and Zhuanchang Wu, and Ke Liu, and Pengfei Qi, and Jinpeng Xu, and Jianchao Wei, and Beibei Li, and Donghua Shao, and Yuanyuan Shi, and Yafeng Qiu, and Zhiyong Ma
The miRNA-targeted transcriptome of porcine alveolar macrophages upon infection with Porcine Reproductive and Respiratory Syndrome Virus.
February 2019, Scientific reports,
Copied contents to your clipboard!