MicroRNA‑141 inhibits epithelial‑mesenchymal transition, and ovarian cancer cell migration and invasion. 2017

Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
Department of Obstetrics and Gynecology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, P.R. China.

The effects of microRNA‑141 (miR‑141) on epithelial‑mesenchymal transition (EMT), and ovarian cancer cell migration and invasion were investigated. SKOV3 cells were transfected with the miR‑141 mimic (mimic group), inhibitor (inhibitor group) and nonspecific sequences (NC group), and left untransfected group (blank group). The reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of miR‑141 in SKOV3 cell lines. Then, mRNA levels and protein expression of EMT markers were determined by RT‑qPCR and western blotting, respectively. Cell proliferation was assessed using an MTT assay, followed by analysis of cell invasion and migration. SPSS software was used for statistical analysis. The results demonstrated that miR‑141 expression in the mimic group was increased compared with the NC or blank group. Compared with the NC or blank group, upregulation of epithelial‑cadherin (E‑cadherin) and integrin‑β, and downregulation of zinc finger E‑box‑binding homeobox (ZEB) was observed in the mimic group. The rate of cell proliferation decreased in the mimic group and increased in the inhibitor group when compared with the NC group (P<0.05). The number of invasive cells significantly increased in the inhibitor group and decreased in the mimic group when compared with the NC group (P<0.01). Compared with the NC group, the migratory rate was decreased in the mimic group, and increased in the inhibitor group at 24 and 48 h (all P<0.01). In conclusion, overexpression of miR‑141 caused upregulation of E‑cadherin, inhibited cell proliferation and EMT, and decreased cell invasion and migration in the SKOV3 cell line.

UI MeSH Term Description Entries
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D002465 Cell Movement The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell. Cell Migration,Locomotion, Cell,Migration, Cell,Motility, Cell,Movement, Cell,Cell Locomotion,Cell Motility,Cell Movements,Movements, Cell
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000070416 Antagomirs Chemically-engineered oligonucleotides used to selectively inhibit expression of target genes through sequence-specific binding of corresponding microRNA (miRNA) sites. Antagomir,Anti-miR,Anti-miRs,Blockmirs,Anti miR,Anti miRs
D000071799 Zinc Finger E-box-Binding Homeobox 1 A transcription factor characterized by N-terminal and C-terminal CYS2-HIS2 ZINC FINGERS separated by a homeobox. It represses the expression of E-CADHERIN to induce the EPITHELIAL-MESENCHYMAL TRANSITION. It also represses PROTO-ONCOGENE PROTEINS C-BCL-6; regulates the cell type-specific expression of SODIUM-POTASSIUM-EXCHANGING ATPASE; and promotes neuronal differentiation. NIL-2-A Zinc Finger Protein,TCF8 Protein,Transcription Factor 8,Zeb1 Transcription Factor,NIL 2 A Zinc Finger Protein,Transcription Factor, Zeb1,Zinc Finger E box Binding Homeobox 1
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D015820 Cadherins Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body. Cadherin,E-Cadherins,Epithelial-Cadherin,Liver Cell Adhesion Molecules,N-Cadherins,Neural Cadherin,P-Cadherins,Uvomorulin,Cadherin-1,Cadherin-2,Cadherin-3,E-Cadherin,Epithelial-Cadherins,Liver Cell Adhesion Molecule,N-Cadherin,Neural Cadherins,P-Cadherin,Placental Cadherins,Cadherin 1,Cadherin 2,Cadherin 3,Cadherin, Neural,Cadherins, Neural,Cadherins, Placental,E Cadherin,E Cadherins,Epithelial Cadherin,Epithelial Cadherins,N Cadherin,N Cadherins,P Cadherin,P Cadherins
D015854 Up-Regulation A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Up-Regulation,Upregulation,Up-Regulation (Physiology),Up Regulation
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines

Related Publications

Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
June 2020, International journal of oncology,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
January 2017, PloS one,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
December 2017, Oncotarget,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
April 2017, The international journal of biochemistry & cell biology,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
September 2019, Molecular and cellular biochemistry,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
October 2017, Oncotarget,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
October 2021, Food & function,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
June 2019, Experimental and therapeutic medicine,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
March 2017, Oncotarget,
Qinghua Ye, and Lei Lei, and Lingyun Shao, and Jing Shi, and Jun Jia, and Xiaowen Tong
January 2013, Oncology reports,
Copied contents to your clipboard!