Catanionic Coacervate Droplets as a Surfactant-Based Membrane-Free Protocell Model. 2017

Jean-Paul Douliez, and Nicolas Martin, and Cédric Gaillard, and Thomas Beneyton, and Jean-Christophe Baret, and Stephen Mann, and Laure Beven
UMR 1332, biologie et pathologie du fruit, INRA, Univ. Bordeaux, centre de Bordeaux, 33883, Villenave d'Ornon, France.

We report on the formation of surfactant-based complex catanionic coacervate droplets in mixtures of decanoic acid and cetylpyridinium chloride or cetyltrimethylammonium bromide. We show that coacervation occurs over a broad range of composition, pH, and ionic strength. The catanionic coacervates consist of elongated micelles, sequester a wide range of solutes including water-soluble organic dyes, polysaccharides, proteins, enzymes, and DNA, and can be structurally stabilized by sodium alginate or gelatin-based hydrogelation. These results suggest that catanionic coacervates could be exploited as a novel surfactant-based membrane-free protocell model.

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