Biomaterial Database

Identification of an intestinal immune response using peripheral blood lymphocytes. 1988

B D Forrest

University of Adelaide, Department of Medicine, Royal Adelaide Hospital, South Australia.

The intestinal immune response of volunteers given the oral vaccine Salmonella typhi Ty21a was assessed with a new immunoassay which measures specific antibody secretion by peripheral blood lymphocytes (PBL). Peak IgA, IgG, and IgM production by PBL occurred on day 7 after the start of vaccination. Peak antibody secretion by PBL occurred several days earlier than the serum antibody peak. All volunteers showed a specific PBL antibody response, but serum or intestinal immune responses, when demonstrable, were highly variable. Peak PBL IgA antibody response correlated with degree of rise in IgA antibody in serum (p = 0.00098) and the intestinal fluid (p = 0.0024). The assay is a useful means of measuring humoral immune response at a mucosal surface after local administration of antigen.

UI	MeSH Term	Description	Entries
D007413	Intestinal Mucosa	Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.	Intestinal Epithelium,Intestinal Glands,Epithelium, Intestinal,Gland, Intestinal,Glands, Intestinal,Intestinal Gland,Mucosa, Intestinal
D008214	Lymphocytes	White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.	Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008297	Male		Males
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260	Female		Females
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328	Adult	A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available.	Adults
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies
D012485	Salmonella typhi	A serotype of SALMONELLA ENTERICA which is the etiologic agent of TYPHOID FEVER.	Salmonella enterica serovar Typhi,Salmonella typhosa
D014436	Typhoid-Paratyphoid Vaccines	Vaccines used to prevent TYPHOID FEVER and/or PARATYPHOID FEVER which are caused by various species of SALMONELLA. Attenuated, subunit, and inactivated forms of the vaccines exist.	Paratyphoid Vaccine,Typhoid Vaccine,Vaccines, Paratyphoid,Vaccines, Typhoid,Paratyphoid Vaccines,Typhoid Paratyphoid Vaccines,Typhoid Vaccines,Vaccine, Paratyphoid,Vaccine, Typhoid,Vaccines, Typhoid-Paratyphoid