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Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA. 1987
D Cross, and
M L Allende, and
R Y Krauss, and
M E Fuentes, and
G Kaltwasser, and
J Alvarez, and
N C Inestrosa
Department of Cell Biology, Faculty of Biological Sciences, School of Medicine, Catholic University of Chile, Santiago.
In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.
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