Characterization of serum anti-diphtheria antibody activity following administration of equine anti-toxin for suspected diphtheria. 2017

Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
a MassBiologics of the University of Massachusetts Medical School , Boston , MA , USA.

There is a global shortage of equine-derived diphtheria anti-toxin (DAT) for diphtheria treatment. There are few existing data on serum antibody concentrations and neutralizing activity post-treatment to support development of new therapeutics. Antibody concentrations were quantified by ELISA and anti-toxin neutralizing activity by cytotoxicity assay in serum from 4 patients receiving DAT for suspected diphtheria. Using linear mixed effects modeling, estimated mean (SE) half-life was 78.2 (20.0) hours. Maximum serum neutralizing activity ranged from 28.42-38.64 AU/mL with an estimated mean AUC1-72 of 1396.7 (399.3) AU/mL*hr. These data provide a standard of comparison for development of novel anti-toxins to replace DAT.

UI MeSH Term Description Entries
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D009500 Neutralization Tests The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). Neutralization Test,Test, Neutralization,Tests, Neutralization
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D003601 Cytotoxicity Tests, Immunologic The demonstration of the cytotoxic effect on a target cell of a lymphocyte, a mediator released by a sensitized lymphocyte, an antibody, or complement. AHG-CDC Tests,Anti-Human Globulin Complement-Dependent Cytotoxicity Tests,Microcytotoxicity Tests,Anti Human Globulin Complement Dependent Cytotoxicity Tests,Anti-Human Globulin Complement-Dependent Cytotoxicity Test,Antiglobulin-Augmented Lymphocytotoxicity Test,Antiglobulin-Augmented Lymphocytotoxicity Tests,Cytotoxicity Test, Immunologic,Cytotoxicity Tests, Anti-Human Globulin Complement-Dependent,Cytotoxicity Tests, Immunological,Immunologic Cytotoxicity Test,Immunologic Cytotoxicity Tests,Lymphocytotoxicity Test, Antiglobulin-Augmented,Lymphocytotoxicity Tests, Antiglobulin-Augmented,Microcytotoxicity Test,AHG CDC Tests,AHG-CDC Test,Anti Human Globulin Complement Dependent Cytotoxicity Test,Antiglobulin Augmented Lymphocytotoxicity Test,Antiglobulin Augmented Lymphocytotoxicity Tests,Cytotoxicity Test, Immunological,Cytotoxicity Tests, Anti Human Globulin Complement Dependent,Immunological Cytotoxicity Test,Immunological Cytotoxicity Tests,Lymphocytotoxicity Test, Antiglobulin Augmented,Lymphocytotoxicity Tests, Antiglobulin Augmented
D004165 Diphtheria A localized infection of mucous membranes or skin caused by toxigenic strains of CORYNEBACTERIUM DIPHTHERIAE. It is characterized by the presence of a pseudomembrane at the site of infection. DIPHTHERIA TOXIN, produced by C. diphtheriae, can cause myocarditis, polyneuritis, and other systemic toxic effects. Corynebacterium diphtheriae Infection,Corynebacterium diphtheriae Infections,Infection, Corynebacterium diphtheriae
D004166 Diphtheria Antitoxin An antitoxin produced against the toxin of CORYNEBACTERIUM DIPHTHERIAE that is used for the treatment of DIPHTHERIA. Antitoxin, Diphtheria
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005260 Female Females
D006207 Half-Life The time it takes for a substance (drug, radioactive nuclide, or other) to lose half of its pharmacologic, physiologic, or radiologic activity. Halflife,Half Life,Half-Lifes,Halflifes

Related Publications

Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
August 2016, Virulence,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
June 1898, The Hospital,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
January 1957, Zhurnal mikrobiologii, epidemiologii i immunobiologii,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
March 1990, The Journal of biological chemistry,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
November 1918, Texas medical journal (Austin, Tex.),
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
November 2000, Journal of immunological methods,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
November 1954, Experimental cell research,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
October 1975, Immunochemistry,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
June 1897, The Hospital,
Heidi L Smith, and Greg Saia, and Maria Lobikin, and Tejpratap Tiwari, and Su-Chun Cheng, and Deborah C Molrine
January 1896, The Homoeopathic physician,
Copied contents to your clipboard!