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Two major aminopeptidases, an aminopeptidase B and an aminopeptidase M-like enzyme, were purified from human skeletal muscle by DEAE-cellulose, HPLC gel filtration, and hydroxyapatite column chromatographies. The purified aminopeptidase B exhibits a molecular weight of 76,000 under both native and denaturing conditions. The activity of the aminopeptidase B is regulated by C1 ions and other anions in vitro. On the other hand, the aminopeptidase M-like enzyme is a monomeric protein having a molecular weight of 96,000. It is capable of significantly cleaving Phe-, Leu-, Arg-, and Ala-aminoacyl bonds in the presence of 2-mercaptoethanol. The pH optima for both enzymes are around 7.0, and bestatin is an effective inhibitor of both enzymes.
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
March 1985,
European journal of biochemistry,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
June 1983,
The Biochemical journal,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
January 1993,
Methods in molecular biology (Clifton, N.J.),
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
February 1991,
Clinica chimica acta; international journal of clinical chemistry,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
June 2018,
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
May 2006,
Bioscience, biotechnology, and biochemistry,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
February 1985,
Acta physiologica Scandinavica,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
April 1992,
Clinica chimica acta; international journal of clinical chemistry,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
October 1988,
Biochimica et biophysica acta,
S Ishiura, and
T Yamamoto, and
M Yamamoto, and
M Nojima, and
T Aoyagi, and
H Sugita
January 1982,
Acta neuropathologica,
