Crosstalk can pose limitations to the applications of surface electromyogram (EMG). Its reduction can help in the identification of the activity of specific muscles. The selectivity of different spatial filters was tested in the literature both in simulations and experiments: their performances are affected by many factors (e.g. anatomy, conduction properties of the tissues and dimension/location of the electrodes); moreover, they reduce crosstalk by decreasing the detection volume, recording data that represent only the activity of a small portion of the muscle of interest. In this study, an alternative idea is proposed, based on a spatio-temporal filter. An adaptive method is applied, which filters both in time and among different channels, providing a signal that maximally preserves the energy of the EMG of interest and discards that of nearby muscles (increasing the signal to crosstalk ratio, SCR). Tests with simulations and experimental data show an average increase of the SCR of about 2 dB with respect to the single or double differential data processed by the filter. This allows to reduce the bias induced by crosstalk in conduction velocity and force estimation. The method can be applied to few channels, so that it is useful in applicative studies (e.g. clinics, gate analysis, rehabilitation protocols with EMG biofeedback and prosthesis control) where limited and not selective information is usually available.