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Acid phosphatase (ACP) was studied in normal and abnormal human semen of individuals not suffering from testosterone deficiency. Study of ACP activity was performed on 365 men referred to the OPD clinic of L.T.M.G. Hospital, Bombay. Semen samples were classified into five groups according to sperm concentration, vitality, motility, and morphology. An inverse correlation was found between enzyme activity and sperm concentration. Statistical analysis revealed that ACP activity was maximal in the azoospermic group, and decreases as the sperm concentration increases. On the basis of this, measurement of ACP in human semen may be useful in clinical practice.
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