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A culture system was devised for the production of the various stages in the developmental cycle of Trypanosoma brucei brucei and T. b. rhodesiense. The bloodstream forms were grown at 37 degrees C on a feeder layer of fibroblasts from embryos of Microtus montanus or CD-1 mice in HEPES-buffered Minimum Essential Medium with Earle's salts, supplemented with 15% heat-inactivated rabbit serum. When they were transferred to HEPES-buffered Cunningham's medium and incubated at 27 degrees C, they transformed into procyclic trypomastigotes, some of which developed into epimastigote and metacyclic forms in the presence of explants of Glossina morsitans or Phormia regina. Stages infective to mice were produced in cultures of procyclic forms grown with Anopheles gambiae cells in medium consisting of a mixture of 3 volumes of Cunningham's medium and 1 volume of Anopheles cell medium. In vitro-produced metacyclic trypanosomes were used to initiate cultures of bloodstream forms.
R Kaminsky, and
E Beaudoin, and
I Cunningham
December 1973,
Parasitology,
R Kaminsky, and
E Beaudoin, and
I Cunningham
October 2015,
The Journal of biological chemistry,
R Kaminsky, and
E Beaudoin, and
I Cunningham
May 1985,
Proceedings of the National Academy of Sciences of the United States of America,
R Kaminsky, and
E Beaudoin, and
I Cunningham
March 1977,
Acta tropica,
R Kaminsky, and
E Beaudoin, and
I Cunningham
January 1973,
Transactions of the Royal Society of Tropical Medicine and Hygiene,
R Kaminsky, and
E Beaudoin, and
I Cunningham
July 2006,
Eukaryotic cell,
R Kaminsky, and
E Beaudoin, and
I Cunningham
November 2022,
The Journal of eukaryotic microbiology,
R Kaminsky, and
E Beaudoin, and
I Cunningham
January 2017,
F1000Research,
R Kaminsky, and
E Beaudoin, and
I Cunningham
January 2020,
Methods in molecular biology (Clifton, N.J.),
R Kaminsky, and
E Beaudoin, and
I Cunningham
January 1977,
Bulletin of the World Health Organization,
