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The binding of L-[3H]glutamate to membranes from human temporal cortex was studied in the absence of Na+, Ca2+, and Cl- ions. Pharmacological characterisation revealed that approximately 35% of specific binding at 50 nM L-[3H]glutamate was sensitive to a combination of kainate and alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid. The remaining approximately 65% of specific binding was to a single population of sites with a KD of 844 nM and a Bmax of 0.92 pmol/mg protein. The pharmacological characteristics were consistent with an interaction at the N-methyl-D-aspartate subclass of excitatory amino acid receptor. The inclusion of Cl- ions revealed additional glutamate binding; this was sensitive to quisqualate and DL-2-amino-4-phosphonobutyrate, but not to kainate, DL-2-amino-7-phosphonoheptanoate, or alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid.
R F Cowburn, and
J A Hardy, and
P J Roberts
January 1991,
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R F Cowburn, and
J A Hardy, and
P J Roberts
November 1986,
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R F Cowburn, and
J A Hardy, and
P J Roberts
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R F Cowburn, and
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P J Roberts
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R F Cowburn, and
J A Hardy, and
P J Roberts
February 1988,
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R F Cowburn, and
J A Hardy, and
P J Roberts
April 1991,
Neuroscience letters,
R F Cowburn, and
J A Hardy, and
P J Roberts
January 1983,
Nature,
R F Cowburn, and
J A Hardy, and
P J Roberts
July 1991,
European journal of pharmacology,
R F Cowburn, and
J A Hardy, and
P J Roberts
August 1990,
Epilepsy research,
R F Cowburn, and
J A Hardy, and
P J Roberts
March 1987,
Biochemical pharmacology,
