Estimating sensitivity of the Kato-Katz technique for the diagnosis of Schistosoma mansoni and hookworm in relation to infection intensity. 2017

Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
Epidemiology and Public Health, Swiss Tropical and Public Health Institute, Basel, Switzerland.

The Kato-Katz technique is the most widely used diagnostic method in epidemiologic surveys and drug efficacy trials pertaining to intestinal schistosomiasis and soil-transmitted helminthiasis. However, the sensitivity of the technique is low, particularly for the detection of light-intensity helminth infections. Examination of multiple stool samples reduces the diagnostic error; yet, most studies rely on a single Kato-Katz thick smear, thus underestimating infection prevalence. We present a model which estimates the sensitivity of the Kato-Katz technique in Schistosoma mansoni and hookworm, as a function of infection intensity for repeated stool sampling and provide estimates of the age-dependent 'true' prevalence. We find that the sensitivity for S. mansoni diagnosis is dominated by missed light infections, which have a low probability to be diagnosed correctly even through repeated sampling. The overall sensitivity strongly depends on the mean infection intensity. In particular at an intensity of 100 eggs per gram of stool (EPG), we estimate a sensitivity of 50% and 80% for one and two samples, respectively. At an infection intensity of 300 EPG, we estimate a sensitivity of 62% for one sample and 90% for two samples. The sensitivity for hookworm diagnosis is dominated by day-to-day variation with typical values for one, two, three, and four samples equal to 50%, 75%, 85%, and 95%, respectively, while it is only weakly dependent on the mean infection intensity in the population. We recommend taking at least two samples and estimate the 'true' prevalence of S. mansoni considering the dependence of the sensitivity on the mean infection intensity and the 'true' hookworm prevalence by taking into account the sensitivity given in the current study.

UI MeSH Term Description Entries
D007223 Infant A child between 1 and 23 months of age. Infants
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D008954 Models, Biological Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment. Biological Model,Biological Models,Model, Biological,Models, Biologic,Biologic Model,Biologic Models,Model, Biologic
D010270 Parasite Egg Count Determination of parasite eggs in feces. Count, Parasite Egg,Counts, Parasite Egg,Egg Count, Parasite,Egg Counts, Parasite,Parasite Egg Counts
D002648 Child A person 6 to 12 years of age. An individual 2 to 5 years old is CHILD, PRESCHOOL. Children
D002675 Child, Preschool A child between the ages of 2 and 5. Children, Preschool,Preschool Child,Preschool Children
D005243 Feces Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006725 Hookworm Infections Infection of humans or animals with hookworms other than those caused by the genus Ancylostoma or Necator, for which the specific terms ANCYLOSTOMIASIS and NECATORIASIS are available. Bunostomiasis,Infections, Hookworm,Bunostomiases,Hookworm Infection,Infection, Hookworm
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths

Related Publications

Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
December 2002, Journal of the Egyptian Society of Parasitology,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
June 2003, The American journal of tropical medicine and hygiene,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
January 2013, Annales de biologie clinique,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
April 2021, PLoS neglected tropical diseases,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
December 1997, The American journal of tropical medicine and hygiene,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
January 1992, Transactions of the Royal Society of Tropical Medicine and Hygiene,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
December 2003, Parasitology,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
September 2015, Parasites & vectors,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
December 2016, Acta tropica,
Oliver Bärenbold, and Giovanna Raso, and Jean T Coulibaly, and Eliézer K N'Goran, and Jürg Utzinger, and Penelope Vounatsou
March 2007, Parasitology international,
Copied contents to your clipboard!