BIOMAT Database Logo BIOMAT Database Logo

Cellular and Subcellular Level Localization of Maize Lipids and Metabolites Using High-Spatial Resolution MALDI Mass Spectrometry Imaging. 2018

Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Department of Chemistry, Iowa State University of Science and Technology, 35A Carver Co-Lab, 1111 Woi Rd., Ames, IA, 50011, USA.

Recent technological advances have pushed the achievable spatial resolution for mass spectrometry imaging (MSI) to cellular and subcellular levels. Direct visualization of maize tissues by this tool has provided key insights into the localization of metabolites and lipids. This chapter outlines methodology for sample preparation, data acquisition, and data analysis of maize tissue sections using high-spatial resolution matrix-assisted laser desorption ionization (MALDI)-MSI, as well as the incorporation of a multi-resolution optical system, which allows for simple inter-conversion between different resolution setups (5, 10, and 50 μm imaging).

UI MeSH Term Description Entries
D007091 Image Processing, Computer-Assisted A technique of inputting two-dimensional or three-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer. Biomedical Image Processing,Computer-Assisted Image Processing,Digital Image Processing,Image Analysis, Computer-Assisted,Image Reconstruction,Medical Image Processing,Analysis, Computer-Assisted Image,Computer-Assisted Image Analysis,Computer Assisted Image Analysis,Computer Assisted Image Processing,Computer-Assisted Image Analyses,Image Analyses, Computer-Assisted,Image Analysis, Computer Assisted,Image Processing, Biomedical,Image Processing, Computer Assisted,Image Processing, Digital,Image Processing, Medical,Image Processings, Medical,Image Reconstructions,Medical Image Processings,Processing, Biomedical Image,Processing, Digital Image,Processing, Medical Image,Processings, Digital Image,Processings, Medical Image,Reconstruction, Image,Reconstructions, Image
D008055 Lipids A generic term for fats and lipoids, the alcohol-ether-soluble constituents of protoplasm, which are insoluble in water. They comprise the fats, fatty oils, essential oils, waxes, phospholipids, glycolipids, sulfolipids, aminolipids, chromolipids (lipochromes), and fatty acids. (Grant & Hackh's Chemical Dictionary, 5th ed) Lipid
D003313 Zea mays A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER. Corn,Indian Corn,Maize,Teosinte,Zea,Corn, Indian
D013347 Subcellular Fractions Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163) Fraction, Subcellular,Fractions, Subcellular,Subcellular Fraction
D053858 Metabolic Networks and Pathways Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites. Metabolic Networks,Metabolic Pathways,Metabolic Network,Metabolic Pathway,Network, Metabolic,Networks, Metabolic,Pathway, Metabolic,Pathways, Metabolic
D055432 Metabolomics The systematic identification and quantitation of all the metabolic products of a cell, tissue, organ, or organism under varying conditions. The METABOLOME of a cell or organism is a dynamic collection of metabolites which represent its net response to current conditions. Metabonomics,Metabolomic,Metabonomic
D019032 Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis. Laser Desorption-Ionization Mass Spectrometry, Matrix-Assisted,MALD-MS,MALDI,Mass Spectrometry, Matrix-Assisted Laser Desorption-Ionization,Mass Spectroscopy, Matrix-Assisted Laser Desorption-Ionization,Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry,Spectroscopy, Mass, Matrix-Assisted Laser Desorption-Ionization,MALDI-MS,MS-MALD,SELDI-TOF-MS,Surface Enhanced Laser Desorption Ionization Mass Spectrometry,Laser Desorption Ionization Mass Spectrometry, Matrix Assisted,MALDI MS,Mass Spectrometry, Matrix Assisted Laser Desorption Ionization,Mass Spectroscopy, Matrix Assisted Laser Desorption Ionization,Matrix Assisted Laser Desorption Ionization Mass Spectrometry

Related Publications

Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Subcellular-level resolution MALDI-MS imaging of maize leaf metabolites by MALDI-linear ion trap-Orbitrap mass spectrometer.
March 2015, Analytical and bioanalytical chemistry,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
High resolution MALDI imaging mass spectrometry of retinal tissue lipids.
August 2014, Journal of the American Society for Mass Spectrometry,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.
October 2023, Nature protocols,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Matrix Recrystallization for MALDI-MS Imaging of Maize Lipids at High-Spatial Resolution.
September 2016, Journal of the American Society for Mass Spectrometry,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
High Spatial Resolution MALDI Imaging Mass Spectrometry of Fresh-Frozen Bone.
February 2022, Analytical chemistry,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Combining Salt Doping and Matrix Sublimation for High Spatial Resolution MALDI Imaging Mass Spectrometry of Neutral Lipids.
October 2019, Analytical chemistry,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Laser beam filtration for high spatial resolution MALDI imaging mass spectrometry.
July 2013, Journal of the American Society for Mass Spectrometry,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
High-mass-resolution MALDI mass spectrometry imaging reveals detailed spatial distribution of metabolites and lipids in roots of barley seedlings in response to salinity stress.
January 2018, Metabolomics : Official journal of the Metabolomic Society,
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
Mapping the Lipids of Skin Sebaceous Glands and Hair Follicles by High Spatial Resolution MALDI Imaging Mass Spectrometry.
March 2022, Pharmaceuticals (Basel, Switzerland),
Maria Emilia Dueñas, and Adam D Feenstra, and Andrew R Korte, and Paige Hinners, and Young Jin Lee
High-mass-resolution MALDI mass spectrometry imaging of metabolites from formalin-fixed paraffin-embedded tissue.
August 2016, Nature protocols,
Copied contents to your clipboard!