-transparent.png){kind=logo}
Effects of the 1-methyl-4-phenylpyridinium ion (MPP+) on DOPA formation and phosphorylation of tyrosine hydroxylase (TH) of rat pheochromocytoma PC12h cells were examined after the cells were cultured with MPP+. DOPA formed from endogenous tyrosine in PC12h cells after a 3-day culture with 100 microM MPP+ was decreased to less than 50% as compared to that in the control cells cultured without MPP+. Kinetical study showed that two apparent forms of TH with different Km existed in the cells cultured with 100 microM MPP+ but one form in that of control. Incorporation of radioactive phosphate into TH molecule was also reduced to 50% of its control value following a 3-day exposure to 100 microM MPP+. These results suggest that MPP+ acutely inhibits the phosphorylation of TH to decrease cellular DOPA formation.
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
January 1987,
Neurochemistry international,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
January 1988,
Life sciences,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
June 1987,
Journal of neurochemistry,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
February 1988,
Neuroscience letters,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
May 2000,
Neurochemistry international,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
April 1988,
Biochemical and biophysical research communications,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
June 1985,
Neuroscience letters,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
August 1991,
Journal of neurochemistry,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
December 1988,
Neuroscience letters,
K Kiuchi, and
M Hagiwara, and
H Hidaka, and
T Nagatsu
September 2002,
European journal of pharmacology,
