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The polymerase chain reaction (PCR) and reverse transcription were used to assess human immunodeficiency virus type 1 (HIV1) RNA expression in peripheral blood mononuclear cell samples from seropositive subjects. HIV RNA was detected from seropositive subjects who had no symptoms, lymphadenopathy syndrome, and acquired immunodeficiency syndrome. DNA PCR of the samples used for RNA extraction showed that seventeen of eighteen (94%) contained HIV proviral DNA. Eleven (65%) of the seventeen DNA-positive samples were also positive for HIV RNA, including samples from four patients undergoing antiviral drug treatment. Serum HIV antigen assays detected only six (32%) of the nineteen PCR-positive samples. Owing to the speed and high sensitivity of PCR for HIV detection, this technique will be suitable for monitoring antiviral therapy and the virus load of people with HIV infections.
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
February 1992,
Journal of virological methods,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
June 1994,
AIDS (London, England),
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
June 1995,
African journal of medicine and medical sciences,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
February 1988,
British journal of haematology,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
August 1997,
AIDS (London, England),
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
December 1993,
Critical reviews in oncology/hematology,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
January 2008,
Journal of the International Academy of Periodontology,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
November 1995,
Immunopharmacology,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
March 1988,
Annales medico-psychologiques,
C Hart, and
G Schochetman, and
T Spira, and
A Lifson, and
J Moore, and
J Galphin, and
J Sninsky, and
C Y Ou
September 1996,
Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association,
