-transparent.png){kind=logo}
A cDNA clone (subclone B) previously isolated from the human parasite Entamoeba histolytica was characterized. DNA sequence analysis of subclone B identified the DNA as that encoding apoferredoxin. E. histolytica ferredoxin cDNA contains unusually short 5' and 3' noncoding regions of 9 and 25 nucleotides, respectively. A genomic ferredoxin clone was isolated from E. histolytica DNA, and comparison of genomic and cDNA sequences revealed that the ferredoxin gene is unspliced. The deduced amino acid sequence of E. histolytica ferredoxin resembles clostridial type of ferredoxins, and shows an arrangement of cysteines characteristic for the coordination of 2[4Fe-4S] centres. Of interest is the absence of an aromatic amino acid in the N-terminal region of the protein, a feature which is conserved in clostridial ferredoxins. Southern blot analysis of three different E. histolytica strains (200:NIH, Rahman and HM-1:IMSS) demonstrated the presence of a family of at least two ferredoxin genes. One of these genes is marked by restriction length polymorphisms in different strains of E. histolytica.
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
February 1980,
Experimental parasitology,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
February 1998,
Journal of biomolecular structure & dynamics,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
June 1996,
Molecular and biochemical parasitology,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
May 1998,
Infection and immunity,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
December 1994,
Molecular and biochemical parasitology,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
January 1997,
Archives of medical research,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
October 1994,
Molecular and biochemical parasitology,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
April 1993,
Nucleic acids research,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
January 1992,
Archives of medical research,
M Huber, and
L Garfinkel, and
C Gitler, and
D Mirelman, and
M Revel, and
S Rozenblatt
January 2024,
Parasitology international,
