BIOMAT Database Logo BIOMAT Database Logo

Identification of N-linked glycosylation sites in the spike protein and their functional impact on the replication and infectivity of coronavirus infectious bronchitis virus in cell culture. 2018

Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
South China Agricultural University, Guangdong Province Key Laboratory Microbial Signals & Disease Co, and Integrative Microbiology Research Centre, Guangzhou 510642, Guangdong, People's Republic of China; School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 63755.

Spike (S) glycoprotein on the viral envelope is the main determinant of infectivity. The S protein of coronavirus infectious bronchitis virus (IBV) contains 29 putative asparagine(N)-linked glycosylation sites. These post-translational modifications may assist in protein folding and play important roles in the functionality of S protein. In this study, we used bioinformatics tools to predict N-linked glycosylation sites and to analyze their distribution in IBV strains and variants. Among these sites, 8 sites were confirmed in the S protein extracted from partially purified virus particles by proteomics approaches. N-D and N-Q substitutions at 13 predicted sites were introduced into an infectious clone system. The impact on S protein-mediated cell-cell fusion, viral recovery and infectivity was assessed, leading to the identification of sites essential for the functions of IBV S protein. Further characterization of these and other uncharacterized sites may reveal novel aspects of N-linked glycosylation in coronavirus replication and pathogenesis.

UI MeSH Term Description Entries
D002522 Chlorocebus aethiops A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research. African Green Monkey,Cercopithecus aethiops,Cercopithecus griseoviridis,Cercopithecus griseus,Cercopithecus pygerythrus,Cercopithecus sabeus,Cercopithecus tantalus,Chlorocebus cynosuros,Chlorocebus cynosurus,Chlorocebus pygerythrus,Green Monkey,Grivet Monkey,Lasiopyga weidholzi,Malbrouck,Malbrouck Monkey,Monkey, African Green,Monkey, Green,Monkey, Grivet,Monkey, Vervet,Savanah Monkey,Vervet Monkey,Savannah Monkey,African Green Monkey,Chlorocebus cynosuro,Green Monkey, African,Green Monkeys,Grivet Monkeys,Malbrouck Monkeys,Malbroucks,Monkey, Malbrouck,Monkey, Savanah,Monkey, Savannah,Savannah Monkeys,Vervet Monkeys
D004252 DNA Mutational Analysis Biochemical identification of mutational changes in a nucleotide sequence. Mutational Analysis, DNA,Analysis, DNA Mutational,Analyses, DNA Mutational,DNA Mutational Analyses,Mutational Analyses, DNA
D006031 Glycosylation The synthetic chemistry reaction or enzymatic reaction of adding carbohydrate or glycosyl groups. GLYCOSYLTRANSFERASES carry out the enzymatic glycosylation reactions. The spontaneous, non-enzymatic attachment of reducing sugars to free amino groups in proteins, lipids, or nucleic acids is called GLYCATION (see MAILLARD REACTION). Protein Glycosylation,Glycosylation, Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001351 Infectious bronchitis virus A species of CORONAVIRUS causing infections in chickens and possibly pheasants. Chicks up to four weeks old are the most severely affected. Avian Infectious Bronchitis Virus,Infectious Bronchitis Virus, Avian,Infectious bronchitis viruses,bronchitis viruses, Infectious
D014709 Vero Cells A CELL LINE derived from the kidney of the African green (vervet) monkey, (CHLOROCEBUS AETHIOPS) used primarily in virus replication studies and plaque assays. Cell, Vero,Cells, Vero,Vero Cell
D014776 Virus Cultivation Process of growing viruses in live animals, plants, or cultured cells. Viral Cultivation,Cultivation, Viral,Cultivation, Virus,Cultivations, Viral,Cultivations, Virus,Viral Cultivations,Virus Cultivations
D014779 Virus Replication The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle. Viral Replication,Replication, Viral,Replication, Virus,Replications, Viral,Replications, Virus,Viral Replications,Virus Replications
D053586 Virus Internalization The entering of cells by viruses following VIRUS ATTACHMENT. This is achieved by ENDOCYTOSIS, by translocation of the whole virus across the cell membrane, by direct MEMBRANE FUSION of the viral membrane with the CELL MEMBRANE, or by fusion of the membrane of infected cells with the membrane of non-infected cells causing SYNCYTIA to be formed. Viral Entry,Viral Internalization,Viral Membrane Fusion,Virus Entry,Virus Membrane Fusion,Entry, Viral,Entry, Virus,Fusion, Viral Membrane,Internalization, Viral,Internalization, Virus,Membrane Fusion, Viral
D019295 Computational Biology A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets. Bioinformatics,Molecular Biology, Computational,Bio-Informatics,Biology, Computational,Computational Molecular Biology,Bio Informatics,Bio-Informatic,Bioinformatic,Biologies, Computational Molecular,Biology, Computational Molecular,Computational Molecular Biologies,Molecular Biologies, Computational

Related Publications

Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
N-glycosylation of infectious bronchitis virus M41 spike determines receptor specificity.
June 2020, The Journal of general virology,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Identification of Important N-Linked Glycosylation Sites in the Hemagglutinin Protein and Their Functional Impact on DC-SIGN Mediated Avian Influenza H5N1 Infection.
January 2021, International journal of molecular sciences,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Cell cycle perturbations induced by infection with the coronavirus infectious bronchitis virus and their effect on virus replication.
April 2006, Journal of virology,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Cryo-EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins.
April 2018, PLoS pathogens,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Lithium chloride inhibits the coronavirus infectious bronchitis virus in cell culture.
April 2007, Avian pathology : journal of the W.V.P.A,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Identification and Comparison of the Sialic Acid-Binding Domain Characteristics of Avian Coronavirus Infectious Bronchitis Virus Spike Protein.
May 2023, Journal of virology,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Isolation of glycoproteins and identification of their N-linked glycosylation sites.
January 2006, Methods in molecular biology (Clifton, N.J.),
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
Mutagenesis of the N-linked glycosylation sites of the yellow fever virus NS1 protein: effects on virus replication and mouse neurovirulence.
August 1996, Virology,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
The neutralization epitopes on the spike protein of infectious bronchitis virus and their antigenic variation.
January 1987, Advances in experimental medicine and biology,
Jie Zheng, and Yoshiyuki Yamada, and To Sing Fung, and Mei Huang, and Raymond Chia, and Ding Xiang Liu
N-Linked glycosylation of the membrane protein ectodomain regulates infectious bronchitis virus-induced ER stress response, apoptosis and pathogenesis.
May 2019, Virology,
Copied contents to your clipboard!