-transparent.png){kind=logo}
Three clones of full-length cDNA encoding human myeloperoxidase were isolated from a human leukemia HL-60 cell cDNA library in lambda gt10 and characterized. Analysis of the nucleotide sequence of one of the cDNA clones, lambda MP-H17, indicated that the cDNA contained 3207 bp with an open reading frame of 2238 bp, a 5' noncoding region of 159 bp, a 3' noncoding region of 800 bp, and a poly(A) tail of 10 bp. cDNA of the two other clones, lambda MP-H7 and lambda MP-H14, each contained insertions with shorter sequences of 96 and 82 bp, respectively, on the open reading frame of lambda MP-H17 cDNA. A myeloperoxidase genomic clone was isolated, and the structure of its 5' region was determined and compared with the structures of these cDNAs. The comparison revealed that the three cDNAs were derived from myeloperoxidase mRNAs produced by alternative splicing from a transcript of the single gene. Nucleotide sequence analysis of the 3' region of the cDNAs of several clones indicated that the mRNAs were polyadenylated at five different sites. Amino acid sequence determination of the amino-terminal and carboxy-terminal portions of the myeloperoxidase light and heavy chains revealed that, during processing of a precursor polypeptide into the mature protein, the amino-terminal polypeptide, the small peptide between the light and heavy chains, and the carboxy-terminal amino acid were excised.
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
August 1992,
Blood,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
December 1997,
The Journal of biological chemistry,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
January 1989,
Nature,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
October 2001,
Gene,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
January 2007,
Annual review of plant biology,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
January 1983,
Journal of molecular biology,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
January 2002,
Genomics,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
February 1997,
Molecular immunology,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
September 2023,
Nature communications,
K Hashinaka, and
C Nishio, and
S J Hur, and
F Sakiyama, and
S Tsunasawa, and
M Yamada
March 2023,
bioRxiv : the preprint server for biology,
