Structure of the ARO3 gene of Saccharomyces cerevisiae. 1988

G Paravicini, and G Braus, and R Hütter
Mikrobiologisches Institut, Eidgenössische Technische Hochschule, ETH-Zentrum, Zürich, Switzerland.

In Saccharomyces cerevisiae, the genes ARO3 and ARO4 encode isoenzymes of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase. Both genes are derepressed seven-fold under the general control of amino acid biosynthesis. A previously isolated 1.7 kb fragment containing the ARO3 gene and the 5'- and 3'-flanking regions was sequenced. The endpoints of the ARO3 transcript coding for a 370 amino acid protein were mapped by primer extension experiments and S1 nuclease digestion. Promoter elements involved in transcription initiation and responsible for the strong general control derepression response are discussed.

UI MeSH Term Description Entries
D007527 Isoenzymes Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics. Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D010649 Phenylalanine An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE. Endorphenyl,L-Phenylalanine,Phenylalanine, L-Isomer,L-Isomer Phenylalanine,Phenylalanine, L Isomer
D010723 3-Deoxy-7-Phosphoheptulonate Synthase An enzyme that catalyzes the formation of 7-phospho-2-keto-3-deoxy-D-arabinoheptonate from phosphoenolpyruvate and D-erythrose-4-phosphate. It is one of the first enzymes in the biosynthesis of TYROSINE and PHENYLALANINE. This enzyme was formerly listed as EC 4.1.2.15. 2-Dehydro-3-Deoxyphosphoheptonate Aldolase,3-Deoxyarabinoheptulosonate-7-Phosphate Synthetase,DAHP Synthase,Phospho-2-Keto-3-Deoxyheptonate Aldolase,3-Deoxy-Arabino-Heptulosonate-7-Phosphate Synthase,3-Deoxy-D-Arabino-Heptulosonate-7-Phosphate Synthase,DAH7-P,2 Dehydro 3 Deoxyphosphoheptonate Aldolase,3 Deoxy 7 Phosphoheptulonate Synthase,3 Deoxy Arabino Heptulosonate 7 Phosphate Synthase,3 Deoxy D Arabino Heptulosonate 7 Phosphate Synthase,3 Deoxyarabinoheptulosonate 7 Phosphate Synthetase,Aldolase, 2-Dehydro-3-Deoxyphosphoheptonate,Aldolase, Phospho-2-Keto-3-Deoxyheptonate,Phospho 2 Keto 3 Deoxyheptonate Aldolase,Synthase, 3-Deoxy-7-Phosphoheptulonate,Synthase, 3-Deoxy-Arabino-Heptulosonate-7-Phosphate,Synthase, 3-Deoxy-D-Arabino-Heptulosonate-7-Phosphate,Synthase, DAHP,Synthetase, 3-Deoxyarabinoheptulosonate-7-Phosphate
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004271 DNA, Fungal Deoxyribonucleic acid that makes up the genetic material of fungi. Fungal DNA
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004720 Endonucleases Enzymes that catalyze the hydrolysis of the internal bonds and thereby the formation of polynucleotides or oligonucleotides from ribo- or deoxyribonucleotide chains. EC 3.1.-. Endonuclease
D005796 Genes A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms. Cistron,Gene,Genetic Materials,Cistrons,Genetic Material,Material, Genetic,Materials, Genetic
D005800 Genes, Fungal The functional hereditary units of FUNGI. Fungal Genes,Fungal Gene,Gene, Fungal

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