Analysis of Polyamines Conjugated with Hydroxycinnamoyl Acids by High-Performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry. 2018

Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
Department of Biology, Healthcare and Environment, Faculty of Pharmacy, University of Barcelona, Avda. Joan XXIII 27-31, 08028, Barcelona, Spain.

Polyamines conjugated with hydroxycinnamic acids are phenolic compounds, which are widespread in the plant kingdom playing important roles in development and defence responses. This chapter describes the methodology employed to analyze these phenolamides in plant material by liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-MS-MS). These compounds are not always in sufficient concentration in plant tissues for analysis by more conventional methods such as UV detection of HPLC. Owing to their particular molecular structure, they cannot be analyzed as free polyamines. Thus, described herein is an extraction method for hydroxycinnamic acid amides in plant tissues such as leaves, and their analysis by LC-MS-MS, including identification and quantification protocols.

UI MeSH Term Description Entries
D010944 Plants Multicellular, eukaryotic life forms of kingdom Plantae. Plants acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations. It is a non-taxonomical term most often referring to LAND PLANTS. In broad sense it includes RHODOPHYTA and GLAUCOPHYTA along with VIRIDIPLANTAE. Plant
D011073 Polyamines Amine compounds that consist of carbon chains or rings containing two or more primary amino groups. Polyamine
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D003373 Coumaric Acids Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants. Coumaric Acid,Hydroxycinnamic Acid,Hydroxycinnamic Acids,Acid, Coumaric,Acid, Hydroxycinnamic,Acids, Coumaric,Acids, Hydroxycinnamic
D015394 Molecular Structure The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds. Structure, Molecular,Molecular Structures,Structures, Molecular
D021241 Spectrometry, Mass, Electrospray Ionization A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry. ESI Mass Spectrometry,Electrospray Ionization Mass Spectrometry,Mass Spectrometry, ESI,Spectrometry, ESI Mass

Related Publications

Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
September 2009, Rapid communications in mass spectrometry : RCM,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
January 2005, Rapid communications in mass spectrometry : RCM,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
April 2005, Journal of analytical toxicology,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
May 2008, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
August 2007, Forensic science international,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
March 2006, Journal of pharmaceutical and biomedical analysis,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
June 2015, Journal of separation science,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
April 1999, Journal of chromatography. B, Biomedical sciences and applications,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
July 2007, Biomedical chromatography : BMC,
Laura Torras-Claveria, and Jaume Bastida, and Francesc Viladomat, and Antonio F Tiburcio
November 2005, Biomedical chromatography : BMC,
Copied contents to your clipboard!