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Expression of receptors for the hypothalamic regulatory peptide, gonadotrophin-releasing hormone (GnRH), was investigated by intracellular recording from Xenopus oocytes injected with poly(A)+ mRNA isolated from rat anterior pituitary glands. Membrane depolarizations were induced in oocytes in a dose-dependent fashion following the application of GnRH (10nM - 1 microM) or a GnRH superactive agonist, buserelin (1nM - 1 microM). The response was reversibly blocked by the addition of a GnRH antagonist (1 microM). TRH (10nM - 1 microM) had no effect on most of these oocytes. In contrast, some other oocytes which showed no responses to GnRH or to the GnRH agonist, displayed depolarizing responses to TRH (10nM - 1 microM). A relatively small number of oocytes responded to both ligands. Control oocytes did not respond to the GnRH analogues or to TRH. This successful expression of the GnRH receptor could provide a new approach to the study of the receptor, and serve as a means for the isolation and cloning of the encoding genes.
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January 1990,
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K A Eidne, and
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S Yoshida
January 1990,
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January 1983,
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K A Eidne, and
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P L Taylor, and
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May 2005,
Journal of neuroendocrinology,
K A Eidne, and
A I McNiven, and
P L Taylor, and
S Plant, and
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D W Lincoln, and
S Yoshida
October 1991,
Proceedings of the National Academy of Sciences of the United States of America,
K A Eidne, and
A I McNiven, and
P L Taylor, and
S Plant, and
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S Yoshida
September 1983,
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K A Eidne, and
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S Plant, and
C R House, and
D W Lincoln, and
S Yoshida
June 1993,
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K A Eidne, and
A I McNiven, and
P L Taylor, and
S Plant, and
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D W Lincoln, and
S Yoshida
November 1988,
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K A Eidne, and
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Neuroscience research,
