-transparent.png){kind=logo}
Arsenite-resistant variants of a trypanosomatid protozoan, Leishmania mexicana amazonensis, were selected in vitro by stepwise increases of sodium arsenite concentrations up to 30 microM in the culture medium. These variants were found to contain amplified DNA as extrachromosomal supercoiled molecules of about 69 kb. They originate from a single chromosome in the wild-type cells. There is evidence of chromosomal changes in these cells associated with the selection for arsenite resistance. The apparent absence of these circular molecules in the wild type and their loss from the drug-sensitive revertants suggest amplification of chromosomal DNA into these extrachromosomal circles as the mechanism of arsenite resistance.
S Detke, and
K Katakura, and
K P Chang
May 1989,
Molecular and biochemical parasitology,
S Detke, and
K Katakura, and
K P Chang
November 1996,
Molecular and biochemical parasitology,
S Detke, and
K Katakura, and
K P Chang
July 1994,
Molecular and biochemical parasitology,
S Detke, and
K Katakura, and
K P Chang
January 2003,
Microbiological research,
S Detke, and
K Katakura, and
K P Chang
November 1997,
Experimental parasitology,
S Detke, and
K Katakura, and
K P Chang
July 2005,
Molecular and biochemical parasitology,
S Detke, and
K Katakura, and
K P Chang
October 1992,
Molecular and biochemical parasitology,
S Detke, and
K Katakura, and
K P Chang
December 2009,
Experimental parasitology,
S Detke, and
K Katakura, and
K P Chang
November 2001,
Experimental parasitology,
S Detke, and
K Katakura, and
K P Chang
June 1997,
The EMBO journal,
