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In a previous paper, we reported the presence of a unique globo-series glycolipid as one of the major neutral glycolipid: Gal alpha 1-3Gal alpha 1-4Gal beta 1-4Glc beta 1-1' Cer, in the subcloned PC 12h pheochromocytoma cells (Ariga, T., Yu, R. K., Scarsdale, J. N., Suzuki, M., Kuroda, Y., Kitagawa, H., and Miyatake, T. (1988) Biochemistry 27, 5335-5340). Recently we found that the subcloned PC 12h cells accumulated other unusual neutral glycolipids. In order to characterize these glycolipids, PC 12h cells were subcutaneously transplanted into rats. The induced tumor tissue accumulated four minor neutral glycolipids, which were purified by droplet counter-current, Iatrobeads column, and preparative thin-layer chromatographies. These glycolipid structures were determined by fast atom bombardment-mass spectrometry, proton nuclear magnetic resonance spectroscopy, permethylation study, and sequential degradation with various exoglycosidases to be as follows: A, Fuc alpha 1-2Gal alpha 1-3Gal alpha 1- 4Gal beta 1-4Glc beta 1-1'Cer; B, GalNAc beta 1-3Gal alpha 1-3Gal alpha 1- 4Gal beta 1-4Glc beta 1-1'Cer; C, Gal alpha 1-3Gal alpha 1-3Gal alpha 1- 4Gal beta 1-4Glc beta 1-1'Cer; and D, Gal alpha 1-3Gal alpha 1-3Gal alpha 1- 3Gal alpha 1-4Gal beta 1-4Glc beta 1-1'Cer. Glycolipids A and B were tentatively characterized in normal rat small intestine (Breimer, M. E., Hansson, G. C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 557-568; Angstrom, J., Breimer, M. E., Falk, K.-E., Hansson, G. C., Karlsson, K.-A., and Leffler, H. (1982) J. Biol. Chem. 257, 682-688). Glycolipids C and D have not been reported in the literature.
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
January 1982,
Advances in experimental medicine and biology,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
July 1994,
International journal of cancer,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
March 1998,
Neurochemical research,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
April 1994,
Archives of biochemistry and biophysics,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
March 1987,
European journal of pharmacology,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
February 1995,
Journal of neurochemistry,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
January 1991,
Chemico-biological interactions,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
December 2005,
Glycobiology,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
January 1987,
Oncogene,
T Ariga, and
M Suzuki, and
R K Yu, and
Y Kuroda, and
I Shimada, and
F Inagaki, and
T Miyatake
August 1988,
FEBS letters,
