Hypervariability, a new phenomenon of genetic instability, related to DNA amplification in Streptomyces ambofaciens. 1989

P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
Laboratoire de Génétique et Microbiologie, Faculté des Sciences de l'Université de Nancy I, France.

The wild-type strain Streptomyces ambofaciens DSM 40697 exhibits a high degree of genetic instability. Pigment-defective colonies were observed in the progeny of wild-type colonies at a frequency of about 0.01. While only 13% of these pigment-defective colonies gave rise to homogeneous progeny exhibiting the mutant parental phenotype, 87% of the mutant colonies gave rise to hetergeneous progeny without a preponderant phenotype. This new phenomenon of instability was called hypervariability. In addition, 21% of the mutant strains arising in hypervariable progeny contained highly reiterated DNA sequences, while amplified DNA sequences could be detected in neither stable pigment-defective mutant clones nor in wild-type clones. These results indicate a frequent association between genetic instability and hypervariability and a frequent association between hypervariability and amplification of DNA sequences.

UI MeSH Term Description Entries
D009154 Mutation Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations. Mutations
D004269 DNA, Bacterial Deoxyribonucleic acid that makes up the genetic material of bacteria. Bacterial DNA
D005784 Gene Amplification A selective increase in the number of copies of a gene coding for a specific protein without a proportional increase in other genes. It occurs naturally via the excision of a copy of the repeating sequence from the chromosome and its extrachromosomal replication in a plasmid, or via the production of an RNA transcript of the entire repeating sequence of ribosomal RNA followed by the reverse transcription of the molecule to produce an additional copy of the original DNA sequence. Laboratory techniques have been introduced for inducing disproportional replication by unequal crossing over, uptake of DNA from lysed cells, or generation of extrachromosomal sequences from rolling circle replication. Amplification, Gene
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D013045 Species Specificity The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species. Species Specificities,Specificities, Species,Specificity, Species
D013302 Streptomyces A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
D014644 Genetic Variation Genotypic differences observed among individuals in a population. Genetic Diversity,Variation, Genetic,Diversity, Genetic,Diversities, Genetic,Genetic Diversities,Genetic Variations,Variations, Genetic
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction

Related Publications

P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
January 1986, Biotechnology & genetic engineering reviews,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
October 1987, Journal of bacteriology,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
June 1992, Gene,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
November 1993, Journal of general microbiology,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
February 1988, Journal of general microbiology,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
November 1993, Journal of general microbiology,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
January 1992, Research in microbiology,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
July 1997, Journal of bacteriology,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
June 1993, Mutation research,
P Leblond, and P Demuyter, and L Moutier, and M Laakel, and B Decaris, and J M Simonet
September 1994, Antimicrobial agents and chemotherapy,
Copied contents to your clipboard!