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Oligonucleotide site-directed mutagenesis was used to introduce a premature stop codon in wildtype A beta k and A alpha k cDNA clones to create truncated A beta k and A alpha k molecules lacking the cytoplasmic domain. Transfected B lymphoma cells expressing an I-Ak molecule with a truncated beta-chain or with truncated alpha- and beta-chains showed profound defects in two Ia-related functions: Ia-restricted Ag presentation and intracytoplasmic signaling. The ability of these transfected cell lines to activate autoreactive T hybrids was markedly impaired whereas loss of Ag presentation to nominal Ag-specific T hybrids was more subtle. Ia-mediated transmembrane signaling as measured by PKC translocation from cytosol to nucleus after stimulation with anti-Ak antibody was greatly affected by truncation of the A beta and A alpha cytoplasmic domains. These results indicate an important role for the highly conserved cytoplasmic domain in Ia-mediated responses.
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
January 1989,
International immunology,
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
April 1995,
International immunology,
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
May 1987,
The American journal of pathology,
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
March 1985,
Journal of immunology (Baltimore, Md. : 1950),
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
January 2002,
Nature immunology,
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
October 1990,
Journal of immunology (Baltimore, Md. : 1950),
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
March 1979,
Investigative ophthalmology & visual science,
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
August 1986,
Proceedings of the National Academy of Sciences of the United States of America,
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
April 1990,
Science (New York, N.Y.),
N Nabavi, and
Z Ghogawala, and
A Myer, and
I J Griffith, and
W F Wade, and
Z Z Chen, and
D J McKean, and
L H Glimcher
February 1987,
Clinical and experimental immunology,
