The C-banding pattern for the karyotype of Paragonimus ohirai representing individuals in a new population is reported. The short arm of chromosome 4 consisted of a large pericentromeric proximal C-band block and euchromatic tip. This pattern has not been observed previously and is designated as type E. Other new observations were: chromosome 5 was composed of pericentromeric heterochromatin, a lightly stained intercalary band at the middle portion of the short arm, and a lightly stained interstitial band at the terminal region of the long arm. Chromosome 7 consisted of pericentromeric heterochromatin and a lightly stained telomeric band at the short arm.