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The effect of protein-free diet to decrease liver activity of branched-chain (-2-oxoacid) dehydrogenase (BCD) complex (active form) and increase BCD kinase activity was unaffected by preparation of hepatocytes, but partially reversed by 25 h of culture of hepatocytes in medium 199. Activation of BCD complex preceded loss of BCD kinase. The effect of culture on BCD complex was completely prevented by omission of branched-chain amino acids and partially prevented by 1 mM-alpha-cyano-4-hydroxycinnamate or 0.2 mM-pyruvate/2 mM-lactate. Protein-free diet decreased plasma branched-chain amino and oxo ('keto') acids and increased plasma pyruvate and lactate. It is concluded: (1) that branched-chain amino acids are involved directly in regulation of activities of BCD complex and BCD kinase; (2) that mitochondrial uptake of branched-chain oxo acids is necessary for regulation of BCD complex activity; and (3) that the stable increase in BCD kinase may function as a hysteresis mechanism.
M Beggs, and
J M Shaw, and
P J Randle
October 1982,
FEBS letters,
M Beggs, and
J M Shaw, and
P J Randle
December 1984,
European journal of biochemistry,
M Beggs, and
J M Shaw, and
P J Randle
July 1982,
FEBS letters,
M Beggs, and
J M Shaw, and
P J Randle
November 1978,
FEBS letters,
M Beggs, and
J M Shaw, and
P J Randle
August 1983,
FEBS letters,
M Beggs, and
J M Shaw, and
P J Randle
April 1981,
FEBS letters,
M Beggs, and
J M Shaw, and
P J Randle
June 1983,
FEBS letters,
M Beggs, and
J M Shaw, and
P J Randle
May 1999,
The Biochemical journal,
M Beggs, and
J M Shaw, and
P J Randle
September 1986,
Diabetes,
M Beggs, and
J M Shaw, and
P J Randle
September 1981,
FEBS letters,
