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Rhythmic potassium transport regulates the circadian clock in human red blood cells. 2017
Erin A Henslee, and
Priya Crosby, and
Stephen J Kitcatt, and
Jack S W Parry, and
Andrea Bernardini, and
Rula G Abdallat, and
Gabriella Braun, and
Henry O Fatoyinbo, and
Esther J Harrison, and
Rachel S Edgar, and
Kai F Hoettges, and
Akhilesh B Reddy, and
Rita I Jabr, and
Malcolm von Schantz, and
John S O'Neill, and
Fatima H Labeed
Department of Mechanical Engineering Sciences, Faculty of Engineering and Physical Sciences, University of Surrey, Guildford, Surrey, GU2 7XH, UK.
Circadian rhythms organize many aspects of cell biology and physiology to a daily temporal program that depends on clock gene expression cycles in most mammalian cell types. However, circadian rhythms are also observed in isolated mammalian red blood cells (RBCs), which lack nuclei, suggesting the existence of post-translational cellular clock mechanisms in these cells. Here we show using electrophysiological and pharmacological approaches that human RBCs display circadian regulation of membrane conductance and cytoplasmic conductivity that depends on the cycling of cytoplasmic K+ levels. Using pharmacological intervention and ion replacement, we show that inhibition of K+ transport abolishes RBC electrophysiological rhythms. Our results suggest that in the absence of conventional transcription cycles, RBCs maintain a circadian rhythm in membrane electrophysiology through dynamic regulation of K+ transport.
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