Biomaterial Database

Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes. 2017

Lukas Kater, and Matthias Thoms, and Clara Barrio-Garcia, and Jingdong Cheng, and Sherif Ismail, and Yasar Luqman Ahmed, and Gert Bange, and Dieter Kressler, and Otto Berninghausen, and Irmgard Sinning, and Ed Hurt, and Roland Beckmann

Gene Center Munich and Center of Integrated Protein Science-Munich (CiPS-M), Department of Biochemistry, Feodor-Lynen-Str. 25, University of Munich, 81377 Munich, Germany.

Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural information, this process is poorly understood. Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 Å to 4.5 Å resolution, thereby providing insights into their sequential folding and assembly pathway. Besides revealing distinct immature rRNA conformations, we map 25 assembly factors in six different assembly states. Notably, the Nsa1-Rrp1-Rpf1-Mak16 module stabilizes the solvent side of the 60S subunit, and the Erb1-Ytm1-Nop7 complex organizes and connects through Erb1's meandering N-terminal extension, eight assembly factors, three ribosomal proteins, and three 25S rRNA domains. Our structural snapshots reveal the order of integration and compaction of the six major 60S domains within early nucleolar 60S particles developing stepwise from the solvent side around the exit tunnel to the central protuberance.

UI	MeSH Term	Description	Entries
D008958	Models, Molecular	Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.	Molecular Models,Model, Molecular,Molecular Model
D002597	Chaetomium	A genus of ascomycetous fungi of the family Chaetomiaceae, order SORDARIALES. Many members are cellulolytic and some mycotoxic. They occur naturally on paper and cotton fabric.	Chaetomiums
D001678	Organelle Biogenesis	The natural growth and development within living CELLS.	Mitochondrial Biogenesis,Biogeneses, Organelle,Biogenesis, Mitochondrial,Biogenesis, Organelle,Organelle Biogeneses
D012261	Ribonucleoproteins	Complexes of RNA-binding proteins with ribonucleic acids (RNA).	Ribonucleoprotein
D053858	Metabolic Networks and Pathways	Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.	Metabolic Networks,Metabolic Pathways,Metabolic Network,Metabolic Pathway,Network, Metabolic,Networks, Metabolic,Pathway, Metabolic,Pathways, Metabolic
D054683	Ribosome Subunits, Large, Eukaryotic	The large subunit of the 80s ribosome of eukaryotes. It is composed of the 28S RIBOSOMAL RNA, the 5.8S RIBOSOMAL RNA, the 5S RIBOSOMAL RNA, and about 50 different RIBOSOMAL PROTEINS.	60S Ribosomal Subunits,60S Ribosome Subunits,Eukaryotic Large Ribosomal Subunits,Eukaryotic Large Ribosome Subunits,Large Ribosomal Subunits, Eukaryotic,Large Ribosome Subunits, Eukaryotic,Ribosomal Subunits, 60S,Ribosomal Subunits, Large, Eukaryotic,Ribosome Subunits, 60S,60S Ribosomal Subunit,60S Ribosome Subunit,Ribosomal Subunit, 60S,Ribosome Subunit, 60S,Subunit, 60S Ribosomal,Subunit, 60S Ribosome,Subunits, 60S Ribosomal,Subunits, 60S Ribosome
D059370	RNA Folding	The processes of RNA tertiary structure formation.	RNA Refolding,RNA Unfolding,Folding, RNA,Foldings, RNA,RNA Foldings,RNA Refoldings,RNA Unfoldings,Refolding, RNA,Refoldings, RNA,Unfolding, RNA,Unfoldings, RNA
D020285	Cryoelectron Microscopy	Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.	Electron Cryomicroscopy,Cryo-electron Microscopy,Cryo electron Microscopy,Cryo-electron Microscopies,Cryoelectron Microscopies,Cryomicroscopies, Electron,Cryomicroscopy, Electron,Electron Cryomicroscopies,Microscopies, Cryo-electron,Microscopies, Cryoelectron,Microscopy, Cryo-electron,Microscopy, Cryoelectron