-transparent.png){kind=logo}
We have purified a human non-pancreatic phospholipase A2 that is present in platelets and is enriched in rheumatoid synovial fluid. The enzyme is calcium-dependent, has a pH optimum of 8-10, and shows a striking preference for substrate presented in the form of Escherichia coli membranes. In the E. coli phospholipase A2 assay the phospholipase exhibits an apparent specific activity of 300 mumol/mg/min. Using oligonucleotide probes based on amino-terminal sequence data, we cloned the corresponding human gene from a genomic DNA library and expressed the gene in animal cells. The protein was secreted from the cells in an active form. The deduced amino acid sequence of the human protein consists of 124 amino acids, contains structural features common to all known phospholipase A2s, and has a half-cystine pattern that is characteristic of the snake venom group II enzymes.
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
February 1991,
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
November 1992,
Seikagaku. The Journal of Japanese Biochemical Society,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
September 1995,
The EMBO journal,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
March 1981,
Biochemistry,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
August 1998,
Biochemical Society transactions,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
July 1983,
Journal of molecular biology,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
June 1995,
Nature structural biology,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
January 1978,
European journal of biochemistry,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
January 1978,
Advances in experimental medicine and biology,
R M Kramer, and
C Hession, and
B Johansen, and
G Hayes, and
P McGray, and
E P Chow, and
R Tizard, and
R B Pepinsky
October 1983,
Clinical chemistry,
