BIOMAT Database Logo BIOMAT Database Logo

Naturally Occurring Frameshift Mutations in the tvb Receptor Gene Are Responsible for Decreased Susceptibility of Chicken to Infection with Avian Leukosis Virus Subgroups B, D, and E. 2018

Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
College of Animal Science, South China Agricultural University and Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, Guangzhou, People's Republic of China.

The group of highly related avian leukosis viruses (ALVs) in chickens are thought to have evolved from a common retroviral ancestor into six subgroups, A to E and J. These ALV subgroups use diverse cellular proteins encoded by four genetic loci in chickens as receptors to gain entry into host cells. Hosts exposed to ALVs might be under selective pressure to develop resistance to ALV infection. Indeed, resistance alleles have previously been identified in all four receptor loci in chickens. The tvb gene encodes a receptor, which determines the susceptibility of host cells to ALV subgroup B (ALV-B), ALV-D, and ALV-E. Here we describe the identification of two novel alleles of the tvb receptor gene, which possess independent insertions each within exon 4. The insertions resulted in frameshift mutations that reveal a premature stop codon that causes nonsense-mediated decay of the mutant mRNA and the production of truncated Tvb protein. As a result, we observed that the frameshift mutations in the tvb gene significantly lower the binding affinity of the truncated Tvb receptors for the ALV-B, ALV-D, and ALV-E envelope glycoproteins and significantly reduce susceptibility to infection by ALV-B, ALV-D and ALV-E in vitro and in vivo Taken together, these findings suggest that frameshift mutation can be a molecular mechanism of reducing susceptibility to ALV and enhance our understanding of virus-host coevolution.IMPORTANCE Avian leukosis virus (ALV) once caused devastating economic loss to the U.S. poultry industry prior the current eradication schemes in place, and it continues to cause severe calamity to the poultry industry in China and Southeast Asia, where deployment of a complete eradication scheme remains a challenge. The tvb gene encodes the cellular receptor necessary for subgroup B, D, and E ALV infection. Two tvb allelic variants that resulted from frameshift mutations have been identified in this study, which have been shown to have significantly reduced functionality in mediating subgroup B, D, and E ALV infection. Unlike the control of herpesvirus-induced diseases by vaccination, the control of avian leukosis in chickens has relied totally on virus eradication measures and host genetic resistance. This finding enriches the allelic pool of the tvb gene and expands the potential for genetic improvement of ALV resistance in varied chicken populations by selection.

UI MeSH Term Description Entries
D011991 Receptors, Virus Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response. Viral Entry Receptor,Viral Entry Receptors,Virus Attachment Factor,Virus Attachment Factors,Virus Attachment Receptor,Virus Attachment Receptors,Virus Entry Receptor,Virus Entry Receptors,Virus Receptor,Virus Receptors,Attachment Factor, Virus,Attachment Factors, Virus,Attachment Receptor, Virus,Attachment Receptors, Virus,Entry Receptor, Viral,Entry Receptor, Virus,Entry Receptors, Viral,Entry Receptors, Virus,Receptor, Viral Entry,Receptor, Virus,Receptor, Virus Attachment,Receptor, Virus Entry,Receptors, Viral Entry,Receptors, Virus Attachment,Receptors, Virus Entry
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001353 Avian Leukosis A group of transmissible viral diseases of chickens and turkeys. Liver tumors are found in most forms, but tumors can be found elsewhere. Leukosis, Avian,Avian Leukoses,Leukoses, Avian
D001354 Avian Leukosis Virus The type species of ALPHARETROVIRUS producing latent or manifest lymphoid leukosis in fowl. Leukosis Virus, Avian,Lymphomatosis Virus, Avian,Rous-Associated Virus,Avian Leukosis Viruses,Avian Lymphomatosis Virus,Avian Lymphomatosis Viruses,Leukosis Viruses, Avian,Lymphomatosis Viruses, Avian,Rous Associated Virus,Virus, Avian Leukosis,Virus, Avian Lymphomatosis,Virus, Rous-Associated,Viruses, Avian Leukosis,Viruses, Avian Lymphomatosis
D016368 Frameshift Mutation A type of mutation in which a number of NUCLEOTIDES deleted from or inserted into a protein coding sequence is not divisible by three, thereby causing an alteration in the READING FRAMES of the entire coding sequence downstream of the mutation. These mutations may be induced by certain types of MUTAGENS or may occur spontaneously. Mutation, Frameshift,Frame Shift Mutation,Out-of-Frame Deletion,Out-of-Frame Insertion,Out-of-Frame Mutation,Deletion, Out-of-Frame,Deletions, Out-of-Frame,Frame Shift Mutations,Frameshift Mutations,Insertion, Out-of-Frame,Insertions, Out-of-Frame,Mutation, Frame Shift,Mutation, Out-of-Frame,Mutations, Frame Shift,Mutations, Frameshift,Mutations, Out-of-Frame,Out of Frame Deletion,Out of Frame Insertion,Out of Frame Mutation,Out-of-Frame Deletions,Out-of-Frame Insertions,Out-of-Frame Mutations
D020022 Genetic Predisposition to Disease A latent susceptibility to disease at the genetic level, which may be activated under certain conditions. Genetic Predisposition,Genetic Susceptibility,Predisposition, Genetic,Susceptibility, Genetic,Genetic Predispositions,Genetic Susceptibilities,Predispositions, Genetic,Susceptibilities, Genetic
D030161 Avian Proteins Proteins obtained from species of BIRDS. Avian Protein,Bird Protein,Bird Proteins,Protein, Avian,Protein, Bird,Proteins, Avian,Proteins, Bird

Related Publications

Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
The CAR1 gene encoding a cellular receptor specific for subgroup B and D avian leukosis viruses maps to the chicken tvb locus.
April 1998, Journal of virology,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
Resistance to infection by subgroups B, D, and E avian sarcoma and leukosis viruses is explained by a premature stop codon within a resistance allele of the tvb receptor gene.
August 2002, Journal of virology,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
A single-amino-acid substitution in the TvbS1 receptor results in decreased susceptibility to infection by avian sarcoma and leukosis virus subgroups B and D and resistance to infection by subgroup E in vitro and in vivo.
March 2008, Journal of virology,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
Acquisition of resistance to avian leukosis virus subgroup B through mutations on tvb cysteine-rich domains in DF-1 chicken fibroblasts.
September 2017, Veterinary research,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
TVB receptors for cytopathic and noncytopathic subgroups of avian leukosis viruses are functional death receptors.
December 2000, Journal of virology,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
Bystander killing during avian leukosis virus subgroup B infection requires TVB(S3) signaling.
December 2003, Journal of virology,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
Naturally occurring hepatitis B virus core gene mutations.
July 1995, Hepatology (Baltimore, Md.),
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
NHE1 gene associated with avian leukosis virus subgroup J infection in chicken.
October 2014, Molecular biology reports,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
Amino acid residues Tyr-67, Asn-72, and Asp-73 of the TVB receptor are important for subgroup E avian sarcoma and leukosis virus interaction.
January 2004, Virology,
Xinjian Li, and Weiguo Chen, and Huanmin Zhang, and Aijun Li, and Dingming Shu, and Hongxing Li, and Zhenkai Dai, and Yiming Yan, and Xinheng Zhang, and Wencheng Lin, and Jingyun Ma, and Qingmei Xie
Two functionally distinct forms of a retroviral receptor explain the nonreciprocal receptor interference among subgroups B, D, and E avian leukosis viruses.
April 2001, Journal of virology,
Copied contents to your clipboard!