Recombinant production of bovine Lactoferrin-derived antimicrobial peptide in tobacco hairy roots expression system. 2018

Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
Department of Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran. Electronic address: m.chahardoli@ilam.ac.ir.

LFchimera is a chimerical peptide containing Lactoferricin and Lactoferrampin antimicrobial peptides of bovine lactoferrin, and it has stronger bactericidal activity. Antimicrobial peptides (AMPs) like LFchimera have great potential as an alternative candidate for conventional antibiotics. Plant hairy roots provide suitable platform for fast, easy and cost-effective production of various recombinant proteins. The aim of this study was to express recombinant LFchimera in Nicotiana tabacum hairy roots and investigate its antimicrobial activity. The integration and expression of the transgene in hairy roots were confirmed by PCR and RT-PCR, respectively. LFchimera levels were quantified by ELISA and the presence of LFchimera was verified by SDS-PAGE analysis of root extracts. Biological activity of the plant derived LFchimera was confirmed by investigating the antimicrobial activity of total solution protein against Escherichia coli (ATCC 8739). Hairy root biomass reached to 4.6 g and LFchimera accumulate about 4.8 μg/g fresh weight in Erlenmeyer flasks.

UI MeSH Term Description Entries
D007781 Lactoferrin An iron-binding protein that was originally characterized as a milk protein. It is widely distributed in secretory fluids and is found in the neutrophilic granules of LEUKOCYTES. The N-terminal part of lactoferrin possesses a serine protease which functions to inactivate the TYPE III SECRETION SYSTEM used by bacteria to export virulence proteins for host cell invasion. Lactotransferrin
D011994 Recombinant Proteins Proteins prepared by recombinant DNA technology. Biosynthetic Protein,Biosynthetic Proteins,DNA Recombinant Proteins,Recombinant Protein,Proteins, Biosynthetic,Proteins, Recombinant DNA,DNA Proteins, Recombinant,Protein, Biosynthetic,Protein, Recombinant,Proteins, DNA Recombinant,Proteins, Recombinant,Recombinant DNA Proteins,Recombinant Proteins, DNA
D002417 Cattle Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor. Beef Cow,Bos grunniens,Bos indicus,Bos indicus Cattle,Bos taurus,Cow,Cow, Domestic,Dairy Cow,Holstein Cow,Indicine Cattle,Taurine Cattle,Taurus Cattle,Yak,Zebu,Beef Cows,Bos indicus Cattles,Cattle, Bos indicus,Cattle, Indicine,Cattle, Taurine,Cattle, Taurus,Cattles, Bos indicus,Cattles, Indicine,Cattles, Taurine,Cattles, Taurus,Cow, Beef,Cow, Dairy,Cow, Holstein,Cows,Dairy Cows,Domestic Cow,Domestic Cows,Indicine Cattles,Taurine Cattles,Taurus Cattles,Yaks,Zebus
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D014026 Nicotiana A plant genus of the family SOLANACEAE. Members contain NICOTINE and other biologically active chemicals; the dried leaves of Nicotiana tabacum are used for SMOKING. Tobacco Plant,Nicotiana tabacum,Plant, Tobacco,Plants, Tobacco,Tobacco Plants
D015870 Gene Expression The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION. Expression, Gene,Expressions, Gene,Gene Expressions
D018517 Plant Roots The usually underground portions of a plant that serve as support, store food, and through which water and mineral nutrients enter the plant. (From American Heritage Dictionary, 1982; Concise Dictionary of Biology, 1990) Plant Bulbs,Plant Root,Bulb, Plant,Bulbs, Plant,Plant Bulb,Root, Plant,Roots, Plant
D030821 Plants, Genetically Modified PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING. Genetically Modified Plants,Plants, Transgenic,Transgenic Plants,GMO Plants,Genetically Engineered Plants,Engineered Plant, Genetically,Engineered Plants, Genetically,GMO Plant,Genetically Engineered Plant,Genetically Modified Plant,Modified Plant, Genetically,Modified Plants, Genetically,Plant, GMO,Plant, Genetically Engineered,Plant, Genetically Modified,Plant, Transgenic,Plants, GMO,Plants, Genetically Engineered,Transgenic Plant

Related Publications

Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
April 2019, Molecular biotechnology,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
April 2019, Molecular biotechnology,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
June 1997, Biotechnology and bioengineering,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
May 2024, Probiotics and antimicrobial proteins,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
January 2012, Methods in molecular biology (Clifton, N.J.),
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
February 2024, Journal of biotechnology,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
June 2016, International journal of molecular sciences,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
April 2001, Protein expression and purification,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
June 2022, Biotechnology and applied biochemistry,
Mahmood Chahardoli, and Arash Fazeli, and Mehdi Ghabooli
March 1998, Biochemistry,
Copied contents to your clipboard!