Antifungal activity of Parastrephia quadrangularis (Meyen) Cabrera extracts against Fusarium verticillioides. 2018

L S Di Ciaccio, and V G Spotorno, and M M Córdoba Estévez, and D J L Ríos, and R H Fortunato, and A E Salvat
Instituto Nacional de Tecnología Agropecuaria, CONICET, Instituto de Patobiología, Prov. de Buenos Aires, Argentina.

Fungi are cosmopolitan organisms that grow in and adapt to a vast number of substrates and environments, and that can cause diseases in humans and animals, as well as in crops. The vast area and diverse geographical characteristics of Argentina, with the consequent climatic diversity make the country an important source of biological resources suitable for the search of new compounds. The aim of the present study was to describe the antifungal activity of extracts of Parastrephia quadrangularis, a species from northern Argentina, against Fusarium verticillioides M7075. Bio-guided fractionation and MS/MS studies were conducted to elucidate the chemical structure of active compounds. The extracts exhibited a minimum inhibitory concentration among 118·74 and 250 μg ml-1 and the differences between the treatments and the inoculum control was 12·5-16·5 mm, respectively, in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue, showed alterations in permeability of plasma membranes. HLPC-MS analysis of active fractions revealed the presence of p-coumaroyloxitremetone, and a derivate structure for another compound is proposed. CONCLUSIONS In Argentina, Fusarium verticillioides causes 'ear rot', a disease that produces important yield and nutritional quality losses in the maize producing region. This study suggests that Parastrephia quadrangularis extracts have potential for the growth inhibition against F. verticillioides M7075, and the bioactivity is reported for the first time. The results obtained will provide a starting point for discover new antimicotic candidate in natural products.

UI MeSH Term Description Entries
D008826 Microbial Sensitivity Tests Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses). Bacterial Sensitivity Tests,Drug Sensitivity Assay, Microbial,Minimum Inhibitory Concentration,Antibacterial Susceptibility Breakpoint Determination,Antibiogram,Antimicrobial Susceptibility Breakpoint Determination,Bacterial Sensitivity Test,Breakpoint Determination, Antibacterial Susceptibility,Breakpoint Determination, Antimicrobial Susceptibility,Fungal Drug Sensitivity Tests,Fungus Drug Sensitivity Tests,Sensitivity Test, Bacterial,Sensitivity Tests, Bacterial,Test, Bacterial Sensitivity,Tests, Bacterial Sensitivity,Viral Drug Sensitivity Tests,Virus Drug Sensitivity Tests,Antibiograms,Concentration, Minimum Inhibitory,Concentrations, Minimum Inhibitory,Inhibitory Concentration, Minimum,Inhibitory Concentrations, Minimum,Microbial Sensitivity Test,Minimum Inhibitory Concentrations,Sensitivity Test, Microbial,Sensitivity Tests, Microbial,Test, Microbial Sensitivity,Tests, Microbial Sensitivity
D010936 Plant Extracts Concentrated pharmaceutical preparations of plants obtained by removing active constituents with a suitable solvent, which is evaporated away, and adjusting the residue to a prescribed standard. Herbal Medicines,Plant Extract,Extract, Plant,Extracts, Plant,Medicines, Herbal
D002463 Cell Membrane Permeability A quality of cell membranes which permits the passage of solvents and solutes into and out of cells. Permeability, Cell Membrane
D003313 Zea mays A plant species of the family POACEAE. It is a tall grass grown for its EDIBLE GRAIN, corn, used as food and animal FODDER. Corn,Indian Corn,Maize,Teosinte,Zea,Corn, Indian
D005670 Fusarium A mitosporic Hypocreales fungal genus, various species of which are important parasitic pathogens of plants and a variety of vertebrates. Teleomorphs include GIBBERELLA. Fusariums
D000935 Antifungal Agents Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues. Anti-Fungal Agents,Antifungal Agent,Fungicides, Therapeutic,Antibiotics, Antifungal,Therapeutic Fungicides,Agent, Antifungal,Anti Fungal Agents,Antifungal Antibiotics
D001118 Argentina Country located in southern South America, bordering the South Atlantic Ocean, between Chile and Uruguay.
D053719 Tandem Mass Spectrometry A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection. Mass Spectrometry-Mass Spectrometry,Mass Spectrometry Mass Spectrometry,Mass Spectrometry, Tandem
D019659 Asteraceae A large plant family of the order Asterales, subclass Asteridae, class Magnoliopsida. The family is also known as Compositae. Flower petals are joined near the base and stamens alternate with the corolla lobes. The common name of "daisy" refers to several genera of this family including Aster; CHRYSANTHEMUM; RUDBECKIA; TANACETUM. Aspilia,Carpesium,Clibadium,Compositae,Doronicum,Emilia Plant,Heterotheca,Iostephane,Leopard's Bane, False,Lychnophora,Phagnalon,Sphaeranthus,Trixis,Viguiera,Enhydra fluctuans,Ixeris,Pseudognaphalium,Silphium,Aspilias,Bane, False Leopard's,Banes, False Leopard's,Carpesiums,Clibadiums,Doronicums,Emilia Plants,Enhydra fluctuan,False Leopard's Bane,False Leopard's Banes,Heterothecas,Iostephanes,Ixeri,Leopard Bane, False,Leopard's Banes, False,Leopards Bane, False,Lychnophoras,Phagnalons,Plant, Emilia,Plants, Emilia,Pseudognaphaliums,Silphiums,Trixi,Viguieras,fluctuan, Enhydra
D025301 Hyphae Microscopic threadlike filaments in FUNGI that are filled with a layer of protoplasm. Collectively, the hyphae make up the MYCELIUM. Hypha,Hyphas

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