Vaccination of healthcare workers against seasonal influenza is recommended to protect staff, their families and patients. This study reports the findings of a survey conducted to explore how to improve uptake of influenza vaccination among staff at a specialist NHS hospital Trust. Responses to the survey were received from 376 members of staff (a 38.6% response rate). Reasons reported by respondents for not being vaccinated included lack of access and beliefs about the efficacy of vaccination. The findings suggest that uptake could be improved by ensuring convenience and flexibility in vaccination provision.