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The Neurospora crassa copper metallothionein gene was cloned and its complete nucleotide sequence is reported. Enriched metallothionein mRNA was used as a template for cDNA synthesis, primed by a metallothionein-specific, synthetic undecanucleotide. The sequence of the cDNA obtained allowed the synthesis of a unique 21-mer which was used to screen a genomic DNA library of N. crassa. In agreement with the published amino acid sequence, the gene codes for a polypeptide 26 amino acid residues in length. The coding region is interrupted by a small intron (94 nucleotides). The gene structure is compared with those of mammalian metallothioneins. In both cases, the coding regions are split by introns, the intron-exon boundaries, however, are in different positions. The neurospora copper metallothionein gene is, to our knowledge, the smallest gene interrupted by an intron isolated so far.
K Münger, and
U A Germann, and
K Lerch
January 1991,
Methods in enzymology,
K Münger, and
U A Germann, and
K Lerch
December 1988,
Journal of biochemistry,
K Münger, and
U A Germann, and
K Lerch
March 1980,
Nature,
K Münger, and
U A Germann, and
K Lerch
January 2005,
FEMS microbiology letters,
K Münger, and
U A Germann, and
K Lerch
January 1979,
Experientia. Supplementum,
K Münger, and
U A Germann, and
K Lerch
June 1991,
Idengaku zasshi,
K Münger, and
U A Germann, and
K Lerch
September 1992,
Current genetics,
K Münger, and
U A Germann, and
K Lerch
October 1989,
The Journal of biological chemistry,
K Münger, and
U A Germann, and
K Lerch
July 2014,
Biochemistry. Biokhimiia,
K Münger, and
U A Germann, and
K Lerch
January 1971,
Journal of general microbiology,
