BIOMAT Database Logo BIOMAT Database Logo

Establishment of practical recellularized liver graft for blood perfusion using primary rat hepatocytes and liver sinusoidal endothelial cells. 2018

Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Tissue decellularization produces a three-dimensional scaffold that can be used to fabricate functional liver grafts following recellularization. Inappropriate cell distribution and clotting during blood perfusion hinder the practical use of recellularized livers. Here we aimed to establish a seeding method for the optimal distribution of parenchymal and endothelial cells, and to evaluate the effect of liver sinusoidal endothelial cells (LSECs) in the decellularized liver. Primary rat hepatocytes and LSECs were seeded into decellularized whole-liver scaffolds via the biliary duct and portal vein, respectively. Biliary duct seeding provided appropriate hepatocyte distribution into the parenchymal space, and portal vein-seeded LSECs simultaneously lined the portal lumen, thereby maintaining function and morphology. Hepatocytes co-seeded with LSECs retained their function compared with those seeded alone. Platelet deposition was significantly decreased and hepatocyte viability was maintained in the co-seeded group after extracorporeal blood perfusion. In conclusion, our seeding method provided optimal cell distribution into the parenchyma and vasculature according to the three-dimensional structure of the decellularized liver. LSECs maintained hepatic function, and supported hepatocyte viability under blood perfusion in the engineered liver graft owing to their antithrombogenicity. This recellularization procedure could help produce practical liver grafts with blood perfusion.

UI MeSH Term Description Entries
D008099 Liver A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances. Livers
D008297 Male Males
D010477 Perfusion Treatment process involving the injection of fluid into an organ or tissue. Perfusions
D011917 Rats, Inbred Lew An inbred strain of rat that is used in BIOMEDICAL RESEARCH. Rats, Inbred Lewis,Rats, Lew,Inbred Lew Rat,Inbred Lew Rats,Inbred Lewis Rats,Lew Rat,Lew Rat, Inbred,Lew Rats,Lew Rats, Inbred,Lewis Rats, Inbred,Rat, Inbred Lew,Rat, Lew
D001769 Blood The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D016031 Liver Transplantation The transference of a part of or an entire liver from one human or animal to another. Grafting, Liver,Hepatic Transplantation,Liver Transplant,Transplantation, Hepatic,Transplantation, Liver,Hepatic Transplantations,Liver Grafting,Liver Transplantations,Liver Transplants,Transplant, Liver
D042783 Endothelial Cells Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer. Capillary Endothelial Cells,Lymphatic Endothelial Cells,Vascular Endothelial Cells,Capillary Endothelial Cell,Cell, Capillary Endothelial,Cell, Endothelial,Cell, Lymphatic Endothelial,Cell, Vascular Endothelial,Cells, Capillary Endothelial,Cells, Endothelial,Cells, Lymphatic Endothelial,Cells, Vascular Endothelial,Endothelial Cell,Endothelial Cell, Capillary,Endothelial Cell, Lymphatic,Endothelial Cell, Vascular,Endothelial Cells, Capillary,Endothelial Cells, Lymphatic,Endothelial Cells, Vascular,Lymphatic Endothelial Cell,Vascular Endothelial Cell
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus

Related Publications

Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion.
February 2018, Journal of visualized experiments : JoVE,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Long-term coculture strategies for primary hepatocytes and liver sinusoidal endothelial cells.
April 2015, Tissue engineering. Part C, Methods,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Generation of Fibrotic Liver Organoids Using Hepatocytes, Primary Liver Sinusoidal Endothelial Cells, Hepatic Stellate Cells, and Macrophages.
October 2023, Cells,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Advanced Method for Isolation of Mouse Hepatocytes, Liver Sinusoidal Endothelial Cells, and Kupffer Cells.
January 2017, Methods in molecular biology (Clifton, N.J.),
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
N-acetylglucosamine-6-phosphate deacetylase in hepatocytes, Kupffer cells and sinusoidal endothelial cells from rat liver.
February 1990, Hepatology (Baltimore, Md.),
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Differential expression of prostanoid receptors in hepatocytes, Kupffer cells, sinusoidal endothelial cells and stellate cells of rat liver.
January 1999, Journal of hepatology,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Trophism and Homeostasis of Liver Sinusoidal Endothelial Graft Cells during Preservation, with and without Hypothermic Oxygenated Perfusion.
September 2022, Biology,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Liver sinusoidal endothelial cells: isolation, purification, characterization and interaction with hepatocytes.
January 1988, Revisiones sobre biologia celular : RBC,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Kupffer cells and not liver sinusoidal endothelial cells prevent lentiviral transduction of hepatocytes.
January 2005, Molecular therapy : the journal of the American Society of Gene Therapy,
Hidenobu Kojima, and Kentaro Yasuchika, and KenIshii Fukumitsu, and Takamichi Ishii, and Satoshi Ogiso, and Yuya Miyauchi, and Ryoya Yamaoka, and Takayuki Kawai, and Hokahiro Katayama, and Elena Yukie Yoshitoshi-Uebayashi, and Sadahiko Kita, and Katsutaro Yasuda, and Naoya Sasaki, and Junji Komori, and Shinji Uemoto
Culture of sinusoidal endothelial cells from rat liver.
August 1998, Journal of gastroenterology and hepatology,
Copied contents to your clipboard!