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Isolated splenic hairy cells from three untreated patients were cultured in presence of recombinant human interferon alpha-2C (IFN alpha). Ultrastructural cytochemistry and immunophenotype analysis with a large panel of monoclonal antibodies were performed to study cellular modification induced by IFN alpha. Hairy cells showed a typical pheno-type: Smlg+, B1+, BA1+/-, anti-Tac+, OKDR+, Leu-M5+, HC2+, TRAP+, myeloperoxidase-. Under our experimental conditions, we found no direct cytotoxic effects or significant variations in morphology, cytochemistry, and percentage of reactivity with the tested monoclonal antibodies. After culturing in the presence of different doses of IFN alpha, we observed a significant enhancement of the expression of class II HLA antigens as demonstrated by increased fluorescence for OKDR, OKla, Leu-10 at fluorescence-activated cell sorting analysis. In agreement with this finding IFN alpha-treated hairy cells showed an increased stimulatory capacity v allogeneic T cells in one-way mixed lymphocyte reaction. To our knowledge this is the first report describing the induction of class II HLA antigens on hairy cells by IFN alpha.
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
January 1984,
European journal of immunology,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
November 1989,
Veterinary immunology and immunopathology,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
January 1984,
Giornale italiano di chemioterapia,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
March 1990,
Clinical and experimental immunology,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
July 1987,
Ophthalmology,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
March 1997,
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
August 1985,
Journal of immunology (Baltimore, Md. : 1950),
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
April 1987,
Tissue antigens,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
March 1991,
Gematologiia i transfuziologiia,
L Baldini, and
A Cortelezzi, and
N Polli, and
A Neri, and
L Nobili, and
A T Maiolo, and
G Lambertenghi-Deliliers, and
E E Polli
March 1987,
Blut,
