BIOMAT Database Logo BIOMAT Database Logo

Molecular testing of Klebsiella pneumoniae contaminating tissue allografts recovered from deceased donors. 2018

Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Microbiological screening of tissue allografts is crucial to prevent the transmission of bacterial and fungal infections to transplant recipients. Klebsiella was the most prevalent and resistant contaminating microorganism observed in our setting in the Iranian Tissue Bank. This study was conducted to determine the presence of extended-spectrum β-lactamase (ESBL) genes, antimicrobial resistance patterns of Klebsiella pneumoniae isolates, and their clonal relationships in allograft materials. K. pneumoniae contaminating bone and other tissue allografts recovered from deceased donors were identified and ESBL isolates were detected using a phenotypic confirmatory method. Antimicrobial susceptibility testing was carried out using the disk diffusion method. Distribution of ESBL genes and molecular typing were performed using polymerase chain reaction (PCR) and Repetitive-element (rep-PCR) methods. Of 3828 donated tissues, 51 (1.3%) were found contaminated by K. pneumoniae isolates. Compared to tissue allografts from brain-dead, heart-beating tissue donors, allografts from donors with circulatory cessation were associated with a higher risk of K. pneumoniae contamination [odds ratio (OR), 1.2 (CI 95% 0.9-2.3) (P value < 0.001)]. Half of the isolates produced ESBL, and the rate of susceptibility to cephalosporins was 51%. Among isolates, 22 (43.1%) harbored CTX-M, 31 (60.8%) SHV, and 9 (17.6%) harbored TEM types. The rep-dendrogram indicated that clones having identical or related strains with a similar antibiotype were isolated in the same period. This study provides evidence that a single clone of K. pneumoniae contaminated tissue allografts recovered from many different donors. A single clone found on tissues from several donors suggests contamination of tissues from a single source such as the tissue recovery process and environment. Genomic DNA testing and clonality of contaminating bacteria using molecular methods can focus the epidemiologic investigation on the tissue allograft recovery process including a search for contamination of the tissue recovery room environment, recovery staff, recovery equipment, reagents, solutions and supplies.

UI MeSH Term Description Entries
D007710 Klebsiella Infections Infections with bacteria of the genus KLEBSIELLA. Infections, Klebsiella,Infection, Klebsiella,Klebsiella Infection
D007711 Klebsiella pneumoniae Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans. Bacillus pneumoniae,Bacterium pneumoniae crouposae,Hyalococcus pneumoniae,Klebsiella pneumoniae aerogenes,Klebsiella rhinoscleromatis
D002102 Cadaver A dead body, usually a human body. Corpse,Cadavers,Corpses
D005798 Genes, Bacterial The functional hereditary units of BACTERIA. Bacterial Gene,Bacterial Genes,Gene, Bacterial
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000900 Anti-Bacterial Agents Substances that inhibit the growth or reproduction of BACTERIA. Anti-Bacterial Agent,Anti-Bacterial Compound,Anti-Mycobacterial Agent,Antibacterial Agent,Antibiotics,Antimycobacterial Agent,Bacteriocidal Agent,Bacteriocide,Anti-Bacterial Compounds,Anti-Mycobacterial Agents,Antibacterial Agents,Antibiotic,Antimycobacterial Agents,Bacteriocidal Agents,Bacteriocides,Agent, Anti-Bacterial,Agent, Anti-Mycobacterial,Agent, Antibacterial,Agent, Antimycobacterial,Agent, Bacteriocidal,Agents, Anti-Bacterial,Agents, Anti-Mycobacterial,Agents, Antibacterial,Agents, Antimycobacterial,Agents, Bacteriocidal,Anti Bacterial Agent,Anti Bacterial Agents,Anti Bacterial Compound,Anti Bacterial Compounds,Anti Mycobacterial Agent,Anti Mycobacterial Agents,Compound, Anti-Bacterial,Compounds, Anti-Bacterial
D001618 beta-Lactamases Enzymes found in many bacteria which catalyze the hydrolysis of the amide bond in the beta-lactam ring. Well known antibiotics destroyed by these enzymes are penicillins and cephalosporins. beta-Lactamase,beta Lactamase,beta Lactamases
D014019 Tissue Donors Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients. Organ Donors,Donors,Ovum Donors,Semen Donors,Transplant Donors,Donor,Donor, Organ,Donor, Ovum,Donor, Semen,Donor, Tissue,Donor, Transplant,Donors, Organ,Donors, Ovum,Donors, Semen,Donors, Tissue,Donors, Transplant,Organ Donor,Ovum Donor,Semen Donor,Tissue Donor,Transplant Donor
D018440 beta-Lactam Resistance Nonsusceptibility of bacteria to the action of the beta-lactam antibiotics. Mechanisms responsible for beta-lactam resistance may be degradation of antibiotics by BETA-LACTAMASES, failure of antibiotics to penetrate, or low-affinity binding of antibiotics to targets. beta-Lactam Resistant,beta-Lactamase Resistance,beta-Lactamase Resistant,Resistance, beta-Lactamase,Resistant, beta-Lactamase,beta Lactam Resistance,beta Lactam Resistant,beta Lactamase Resistance,beta Lactamase Resistant
D024881 Drug Resistance, Bacterial The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS). Antibiotic Resistance, Bacterial,Antibacterial Drug Resistance

Related Publications

Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Effective serological and molecular screening of deceased tissue donors.
December 2013, Cell and tissue banking,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals.
December 2016, Scientific reports,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Clonal complexes of carbapenem-resistant Klebsiella pneumoniae recovered from community sewage.
January 2023, Journal of water and health,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Glomerular function, structure, and number in renal allografts from older deceased donors.
January 2009, Journal of the American Society of Nephrology : JASN,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Expression of complement components differs between kidney allografts from living and deceased donors.
August 2009, Journal of the American Society of Nephrology : JASN,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples.
August 2021, AMB Express,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Phenotypic and genotypic characterization of Klebsiella pneumoniae isolates recovered from nonhuman primates.
July 2012, Journal of wildlife diseases,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
[Molecular characteristics of beta-lactamase from Klebsiella pneumoniae].
February 2002, Zhonghua yi xue za zhi,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Molecular pathogenesis of Klebsiella pneumoniae.
January 2014, Future microbiology,
Zohreh Ghalavand, and Alireza Heidary Rouchi, and Hassan Bahraminasab, and Elham Ravanasa, and Elnaz Sadat Mirsamadi, and Narges Nodeh Farahani, and Bahram Nikmanesh
Molecular epidemiology of Klebsiella pneumoniae.
August 1991, Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology,
Copied contents to your clipboard!