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Iron uptake by Escherichia coli under aerobic conditions of iron deficiency is mediated by a highly stable ferric enterobactin [Fe(ent)3-] siderophore complex. Mössbauer spectroscopy has been used to monitor the fate of the iron as 57Fe(ent) was taken up by the cells. Osmotic shock experiments were used to distinguish between the iron present in the periplasmic space and that in the cytoplasm of the cell. Iron delivery by a synthetic analog of enterobactin, 1,3,5-N,N',N''- tris-(2,3-dihydroxybenzoyl)triaminomethylbenzene (MECAM), was also studied. Although Fe-MECAM was transported at the same rate as was Fe(ent) across the outer membrane and was apparently accumulated in the periplasmic space, the subsequent behaviors of Fe(ent) and Fe-MECAM were very different. After more than 30 min, a major fraction of the iron originally absorbed as ferric enterobactin appeared as Fe(II), apparently in the cytoplasm of the cell. However, little iron was delivered to the cytoplasm by the MECAM complex. The differences in specificity of these two stages of iron uptake by E. coli are discussed.
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
August 1989,
European journal of biochemistry,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
June 1980,
Biochimica et biophysica acta,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
March 1999,
Biochimie,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
September 2003,
Frontiers in bioscience : a journal and virtual library,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
November 1984,
Journal of bacteriology,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
April 1972,
Biochimica et biophysica acta,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
December 2008,
Journal of biological physics,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
June 1979,
FEBS letters,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
November 1990,
Journal of bacteriology,
B F Matzanke, and
D J Ecker, and
T S Yang, and
B H Huynh, and
G Müller, and
K N Raymond
January 1980,
Journal of agricultural and food chemistry,
