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PHA stimulation assay was the first in vitro method for evaluating the T-cell function, and this T-cell proliferative response has been routinely used to discriminate between normal subjects and patients with deficiency in cell-mediated immunity. However, [3H]thymidine incorporation into lymphocyte DNA can be studied by using additional in vitro assay methods since they measure different lymphocyte activation pathways. In the present study we selected three different tests to investigate the reliability of this single approach: PHA induced lymphocyte DNA synthesis; T lymphocyte DNA synthesis to anti-T3 monoclonal antibody (OKT3); autologous mixed lymphocyte reaction (AMLR). In addition, IL-2 receptor expression on the membrane of T-cell stimulated in AMLR both with PHA and anti-T3 was evaluated. This study was performed in various groups of subjects: normal young controls, aged healthy individuals, and patients with Alzheimer's disease (AD), Retinitis Pigmentosa (RP), and with cell-mediated immunodeficiency and clinical evidence of recurrent viral infections (ID). The data reported herein show heterogeneity of results in each group studied and demonstrate the necessity of employing more than one laboratory test for the routine evaluation of T-cell-mediated immunity.
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
April 1973,
Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
September 1975,
Clinical oncology,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
January 1989,
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
June 1980,
Clinical oncology,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
January 1988,
Clinical immunology and immunopathology,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
March 1983,
Radiation research,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
November 1971,
Cell and tissue kinetics,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
November 1987,
Gan to kagaku ryoho. Cancer & chemotherapy,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
January 1983,
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie,
W Dirienzo, and
G F Stefanini, and
V K Singh, and
E E Paulling, and
G W Canonica, and
H H Fudenberg
May 2001,
Pediatric radiology,
