Biomaterial Database

Processing of DNA Double-Strand Breaks in Yeast. 2018

Robert Gnügge, and Julyun Oh, and Lorraine S Symington

Columbia University Medical Center, New York, NY, United States.

Single-stranded DNA (ssDNA) intermediates are essential for homology-dependent repair of DNA double-strand breaks (DSBs) and for the DNA damage response. Here we describe methods routinely used to identify ssDNA intermediates formed by end processing of site-specific DSBs in Saccharomyces cerevisiae. These methods have been applied in other model systems and human cell lines, and are useful tools to gain insight into the enzymes that process DSBs and how they are regulated.

UI	MeSH Term	Description	Entries
D009693	Nucleic Acid Hybridization	Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)	Genomic Hybridization,Acid Hybridization, Nucleic,Acid Hybridizations, Nucleic,Genomic Hybridizations,Hybridization, Genomic,Hybridization, Nucleic Acid,Hybridizations, Genomic,Hybridizations, Nucleic Acid,Nucleic Acid Hybridizations
D004271	DNA, Fungal	Deoxyribonucleic acid that makes up the genetic material of fungi.	Fungal DNA
D004277	DNA, Single-Stranded	A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.	Single-Stranded DNA,DNA, Single Stranded,Single Stranded DNA
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D015739	Nocodazole	Nocodazole is an antineoplastic agent which exerts its effect by depolymerizing microtubules.	NSC-238159,Oncodazole,R-17934,R17934,NSC 238159,NSC238159,R 17934
D053903	DNA Breaks, Double-Stranded	Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently.	Double-Stranded DNA Breaks,Double-Strand DNA Breaks,Double-Stranded DNA Break,Break, Double-Strand DNA,Break, Double-Stranded DNA,Breaks, Double-Strand DNA,Breaks, Double-Stranded DNA,DNA Break, Double-Strand,DNA Break, Double-Stranded,DNA Breaks, Double Stranded,DNA Breaks, Double-Strand,Double Strand DNA Breaks,Double Stranded DNA Break,Double Stranded DNA Breaks,Double-Strand DNA Break
D059565	G2 Phase Cell Cycle Checkpoints	CELL CYCLE regulatory signaling systems that are triggered by DNA DAMAGE or lack of nutrients during G2 PHASE. When triggered they restrain cells transitioning from G2 phase to M PHASE.	Antephase Checkpoint,G2 Cell Cycle Arrest,G2 Cell Cycle Checkpoints,G2 Cell Cycle Control,G2 Phase Checkpoints,G2 Restriction Point,G2-M Cell Cycle Checkpoint,Antephase Checkpoints,Checkpoint, Antephase,Checkpoint, G2 Phase,Checkpoints, Antephase,Checkpoints, G2 Phase,G2 M Cell Cycle Checkpoint,G2 Phase Checkpoint,G2 Restriction Points,Restriction Point, G2,Restriction Points, G2
D059767	Recombinational DNA Repair	Repair of DNA DAMAGE by exchange of DNA between matching sequences, usually between the allelic DNA (ALLELES) of sister chromatids.	Homologous Recombination DNA Repair,Homologous Recombination Repair of DNA,Homology-Directed dsDNA Break Repair,Homologous Recombination Double-Stranded Break DNA Repair,Homologous Recombination Repair,Homologous Recombinational Repair,Recombination Repair,Recombinational Repair of DNA,DNA Recombinational Repair,DNA Repair, Recombinational,Homologous Recombination Double Stranded Break DNA Repair,Homologous Recombinational Repairs,Homology Directed dsDNA Break Repair,Recombination Repair, Homologous,Recombinational Repair, Homologous,Repair, Homologous Recombinational,Repair, Recombination,Repair, Recombinational DNA
D060888	Real-Time Polymerase Chain Reaction	Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.	Kinetic Polymerase Chain Reaction,Quantitative Real-Time PCR,Quantitative Real-Time Polymerase Chain Reaction,Real-Time PCR,PCR, Quantitative Real-Time,PCR, Real-Time,PCRs, Quantitative Real-Time,PCRs, Real-Time,Quantitative Real Time PCR,Quantitative Real Time Polymerase Chain Reaction,Quantitative Real-Time PCRs,Real Time PCR,Real Time Polymerase Chain Reaction,Real-Time PCR, Quantitative,Real-Time PCRs,Real-Time PCRs, Quantitative
D029701	Saccharomyces cerevisiae Proteins	Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.	Baker's Yeast Proteins,S cerevisiae Proteins