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A study on the extent of 18O incorporation into various hydroxy-eicosatetraenoic acids, thromboxane B2 and 2,3-dinor-thromboxane B2 by base-catalysed hydrolysis of their methyl ester derivatives is presented. The amount as well as the position of the inbuilt 18O atoms were determined by gas chromatography/mass spectrometry, utilizing electron impact and positive and negative ion chemical ionization.
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